Controlled adsorption of cellulase onto pretreated corncob by pH adjustment

The effective recycling of cellulase requires an in-depth understanding of cellulase adsorption and desorption. In the present study, we examined the adsorption behaviors and stabilities of cellulase at different pH values. Acidic pH ( 0.96) the pseudo-first-order kinetic and Langmuir adsorption iso...

Full description

Saved in:
Bibliographic Details
Published in:Cellulose (London) 2012-04, Vol.19 (2), p.371-380
Main Authors: Du, Ruoyu, Su, Rongxin, Li, Xuan, Tantai, Xiaowei, Liu, Zhaohui, Yang, Jifeng, Qi, Wei, He, Zhimin
Format: Article
Language:English
Subjects:
Adsorption
Bioorganic Chemistry
Cellulase
Ceramics
Chemistry
Chemistry and Materials Science
Composites
Deactivation
Desorption
Glass
Isotherms
Natural Materials
Organic Chemistry
Physical Chemistry
Polymer Sciences
Substrates
Sustainable Development
Temperature
Temperature dependence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c316t-cb50619f636b1f6a1cd76c21b2e843c992054e849f1d9c442a8decbf644c1353
cites cdi_FETCH-LOGICAL-c316t-cb50619f636b1f6a1cd76c21b2e843c992054e849f1d9c442a8decbf644c1353
container_end_page 380
container_issue 2
container_start_page 371
container_title Cellulose (London)
container_volume 19
creator Du, Ruoyu
Su, Rongxin
Li, Xuan
Tantai, Xiaowei
Liu, Zhaohui
Yang, Jifeng
Qi, Wei
He, Zhimin
description The effective recycling of cellulase requires an in-depth understanding of cellulase adsorption and desorption. In the present study, we examined the adsorption behaviors and stabilities of cellulase at different pH values. Acidic pH ( 0.96) the pseudo-first-order kinetic and Langmuir adsorption isotherm ( R 2  > 0.99) models.
doi_str_mv 10.1007/s10570-012-9653-0
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2259911761</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2259911761</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-cb50619f636b1f6a1cd76c21b2e843c992054e849f1d9c442a8decbf644c1353</originalsourceid><addsrcrecordid>eNp1kLFOwzAQQC0EEqXwAWyRmA13TuzEI6qAIiqxdGCzHMdGrdI42M7Qv8dVkDox3Q3v3UmPkHuERwSonyICr4ECMioFLylckAXymtGmYV-XZAFSSAqslNfkJsY9AMia4YJ8rPyQgu972xW6iz6MaeeHwrvC2L6feh1tkQlfjMGmYHXKnPFhML4t2mMxrrO1n2I62CHdkiun-2jv_uaSbF9ftqs13Xy-va-eN9SUKBI1LQeB0olStOiERtPVwjBsmW2q0kjJgFd5lQ47aaqK6aazpnWiqgyWvFySh_nsGPzPZGNSez-FIX9UjHEpEWuBmcKZMsHHGKxTY9gddDgqBHVKpuZkKidTp2QKssNmJ2Z2-LbhfPl_6Rek0G7B</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2259911761</pqid></control><display><type>article</type><title>Controlled adsorption of cellulase onto pretreated corncob by pH adjustment</title><source>Springer Link</source><creator>Du, Ruoyu ; Su, Rongxin ; Li, Xuan ; Tantai, Xiaowei ; Liu, Zhaohui ; Yang, Jifeng ; Qi, Wei ; He, Zhimin</creator><creatorcontrib>Du, Ruoyu ; Su, Rongxin ; Li, Xuan ; Tantai, Xiaowei ; Liu, Zhaohui ; Yang, Jifeng ; Qi, Wei ; He, Zhimin</creatorcontrib><description>The effective recycling of cellulase requires an in-depth understanding of cellulase adsorption and desorption. In the present study, we examined the adsorption behaviors and stabilities of cellulase at different pH values. Acidic pH (&lt;4.8) was found to favor adsorption, whereas neutral and alkaline pH (especially pH 7 and 10) favored desorption. The influence of pH on cellulase activity was temperature dependent. Under mild conditions (e.g., pH 7 and 25 °C), the effect of pH on cellulase activity was reversible, and the cellulase activity can return to almost 100% by adjusting the pH value to 4.8. However, under severe conditions (e.g. pH 10 and 50 °C), irreversible inactivation may take place. We also explored the roles of pH and temperature in cellulase adsorption kinetics and isotherms. At pH 4.8, temperature had no remarkable effect on the adsorption capacity of the cellulases onto substrate. However, at pH 7 and 10, high temperatures lead to more cellulase desorption. Only at pH 4.8 does cellulase adsorption well fit ( R 2  &gt; 0.96) the pseudo-first-order kinetic and Langmuir adsorption isotherm ( R 2  &gt; 0.99) models.</description><identifier>ISSN: 0969-0239</identifier><identifier>EISSN: 1572-882X</identifier><identifier>DOI: 10.1007/s10570-012-9653-0</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adsorption ; Bioorganic Chemistry ; Cellulase ; Ceramics ; Chemistry ; Chemistry and Materials Science ; Composites ; Deactivation ; Desorption ; Glass ; Isotherms ; Natural Materials ; Organic Chemistry ; Physical Chemistry ; Polymer Sciences ; Substrates ; Sustainable Development ; Temperature ; Temperature dependence</subject><ispartof>Cellulose (London), 2012-04, Vol.19 (2), p.371-380</ispartof><rights>Springer Science+Business Media B.V. 2012</rights><rights>Cellulose is a copyright of Springer, (2012). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-cb50619f636b1f6a1cd76c21b2e843c992054e849f1d9c442a8decbf644c1353</citedby><cites>FETCH-LOGICAL-c316t-cb50619f636b1f6a1cd76c21b2e843c992054e849f1d9c442a8decbf644c1353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Du, Ruoyu</creatorcontrib><creatorcontrib>Su, Rongxin</creatorcontrib><creatorcontrib>Li, Xuan</creatorcontrib><creatorcontrib>Tantai, Xiaowei</creatorcontrib><creatorcontrib>Liu, Zhaohui</creatorcontrib><creatorcontrib>Yang, Jifeng</creatorcontrib><creatorcontrib>Qi, Wei</creatorcontrib><creatorcontrib>He, Zhimin</creatorcontrib><title>Controlled adsorption of cellulase onto pretreated corncob by pH adjustment</title><title>Cellulose (London)</title><addtitle>Cellulose</addtitle><description>The effective recycling of cellulase requires an in-depth understanding of cellulase adsorption and desorption. In the present study, we examined the adsorption behaviors and stabilities of cellulase at different pH values. Acidic pH (&lt;4.8) was found to favor adsorption, whereas neutral and alkaline pH (especially pH 7 and 10) favored desorption. The influence of pH on cellulase activity was temperature dependent. Under mild conditions (e.g., pH 7 and 25 °C), the effect of pH on cellulase activity was reversible, and the cellulase activity can return to almost 100% by adjusting the pH value to 4.8. However, under severe conditions (e.g. pH 10 and 50 °C), irreversible inactivation may take place. We also explored the roles of pH and temperature in cellulase adsorption kinetics and isotherms. At pH 4.8, temperature had no remarkable effect on the adsorption capacity of the cellulases onto substrate. However, at pH 7 and 10, high temperatures lead to more cellulase desorption. Only at pH 4.8 does cellulase adsorption well fit ( R 2  &gt; 0.96) the pseudo-first-order kinetic and Langmuir adsorption isotherm ( R 2  &gt; 0.99) models.</description><subject>Adsorption</subject><subject>Bioorganic Chemistry</subject><subject>Cellulase</subject><subject>Ceramics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Deactivation</subject><subject>Desorption</subject><subject>Glass</subject><subject>Isotherms</subject><subject>Natural Materials</subject><subject>Organic Chemistry</subject><subject>Physical Chemistry</subject><subject>Polymer Sciences</subject><subject>Substrates</subject><subject>Sustainable Development</subject><subject>Temperature</subject><subject>Temperature dependence</subject><issn>0969-0239</issn><issn>1572-882X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp1kLFOwzAQQC0EEqXwAWyRmA13TuzEI6qAIiqxdGCzHMdGrdI42M7Qv8dVkDox3Q3v3UmPkHuERwSonyICr4ECMioFLylckAXymtGmYV-XZAFSSAqslNfkJsY9AMia4YJ8rPyQgu972xW6iz6MaeeHwrvC2L6feh1tkQlfjMGmYHXKnPFhML4t2mMxrrO1n2I62CHdkiun-2jv_uaSbF9ftqs13Xy-va-eN9SUKBI1LQeB0olStOiERtPVwjBsmW2q0kjJgFd5lQ47aaqK6aazpnWiqgyWvFySh_nsGPzPZGNSez-FIX9UjHEpEWuBmcKZMsHHGKxTY9gddDgqBHVKpuZkKidTp2QKssNmJ2Z2-LbhfPl_6Rek0G7B</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Du, Ruoyu</creator><creator>Su, Rongxin</creator><creator>Li, Xuan</creator><creator>Tantai, Xiaowei</creator><creator>Liu, Zhaohui</creator><creator>Yang, Jifeng</creator><creator>Qi, Wei</creator><creator>He, Zhimin</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20120401</creationdate><title>Controlled adsorption of cellulase onto pretreated corncob by pH adjustment</title><author>Du, Ruoyu ; Su, Rongxin ; Li, Xuan ; Tantai, Xiaowei ; Liu, Zhaohui ; Yang, Jifeng ; Qi, Wei ; He, Zhimin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-cb50619f636b1f6a1cd76c21b2e843c992054e849f1d9c442a8decbf644c1353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adsorption</topic><topic>Bioorganic Chemistry</topic><topic>Cellulase</topic><topic>Ceramics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>Deactivation</topic><topic>Desorption</topic><topic>Glass</topic><topic>Isotherms</topic><topic>Natural Materials</topic><topic>Organic Chemistry</topic><topic>Physical Chemistry</topic><topic>Polymer Sciences</topic><topic>Substrates</topic><topic>Sustainable Development</topic><topic>Temperature</topic><topic>Temperature dependence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Ruoyu</creatorcontrib><creatorcontrib>Su, Rongxin</creatorcontrib><creatorcontrib>Li, Xuan</creatorcontrib><creatorcontrib>Tantai, Xiaowei</creatorcontrib><creatorcontrib>Liu, Zhaohui</creatorcontrib><creatorcontrib>Yang, Jifeng</creatorcontrib><creatorcontrib>Qi, Wei</creatorcontrib><creatorcontrib>He, Zhimin</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Cellulose (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Ruoyu</au><au>Su, Rongxin</au><au>Li, Xuan</au><au>Tantai, Xiaowei</au><au>Liu, Zhaohui</au><au>Yang, Jifeng</au><au>Qi, Wei</au><au>He, Zhimin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Controlled adsorption of cellulase onto pretreated corncob by pH adjustment</atitle><jtitle>Cellulose (London)</jtitle><stitle>Cellulose</stitle><date>2012-04-01</date><risdate>2012</risdate><volume>19</volume><issue>2</issue><spage>371</spage><epage>380</epage><pages>371-380</pages><issn>0969-0239</issn><eissn>1572-882X</eissn><abstract>The effective recycling of cellulase requires an in-depth understanding of cellulase adsorption and desorption. In the present study, we examined the adsorption behaviors and stabilities of cellulase at different pH values. Acidic pH (&lt;4.8) was found to favor adsorption, whereas neutral and alkaline pH (especially pH 7 and 10) favored desorption. The influence of pH on cellulase activity was temperature dependent. Under mild conditions (e.g., pH 7 and 25 °C), the effect of pH on cellulase activity was reversible, and the cellulase activity can return to almost 100% by adjusting the pH value to 4.8. However, under severe conditions (e.g. pH 10 and 50 °C), irreversible inactivation may take place. We also explored the roles of pH and temperature in cellulase adsorption kinetics and isotherms. At pH 4.8, temperature had no remarkable effect on the adsorption capacity of the cellulases onto substrate. However, at pH 7 and 10, high temperatures lead to more cellulase desorption. Only at pH 4.8 does cellulase adsorption well fit ( R 2  &gt; 0.96) the pseudo-first-order kinetic and Langmuir adsorption isotherm ( R 2  &gt; 0.99) models.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10570-012-9653-0</doi><tpages>10</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0969-0239
ispartof Cellulose (London), 2012-04, Vol.19 (2), p.371-380
issn 0969-0239
1572-882X
language eng
recordid cdi_proquest_journals_2259911761
source Springer Link
subjects Adsorption
Bioorganic Chemistry
Cellulase
Ceramics
Chemistry
Chemistry and Materials Science
Composites
Deactivation
Desorption
Glass
Isotherms
Natural Materials
Organic Chemistry
Physical Chemistry
Polymer Sciences
Substrates
Sustainable Development
Temperature
Temperature dependence
title Controlled adsorption of cellulase onto pretreated corncob by pH adjustment
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T13%3A16%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Controlled%20adsorption%20of%20cellulase%20onto%20pretreated%20corncob%20by%20pH%20adjustment&rft.jtitle=Cellulose%20(London)&rft.au=Du,%20Ruoyu&rft.date=2012-04-01&rft.volume=19&rft.issue=2&rft.spage=371&rft.epage=380&rft.pages=371-380&rft.issn=0969-0239&rft.eissn=1572-882X&rft_id=info:doi/10.1007/s10570-012-9653-0&rft_dat=%3Cproquest_cross%3E2259911761%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c316t-cb50619f636b1f6a1cd76c21b2e843c992054e849f1d9c442a8decbf644c1353%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2259911761&rft_id=info:pmid/&rfr_iscdi=true