Control of properties of nanocomposites bio-based collagen and cellulose nanocrystals

• Published in: Cellulose (London) 2017-04, Vol.24 (4), p.1731-1744
• Main Authors: Rodrigues, Ana P. H., Pereira, Iaci M., de Souza, Sâmara D., Gil, Camila S. Brey, Machado, Giovanna, Carvalho, Sandhra M., Pereira, Fabiano V., Paiva, Paulo R. P., de Oliveira, Luiz C. A., de O. Patricio, Patrícia S.
• Format: Article
• Language: English
• Subjects: Biocompatibility; Biomedical materials; Bioorganic Chemistry; Cellulose; Ceramics; Chemistry; Chemistry and Materials Science; Collagen; Composites; Contact angle; Fillers; Glass; Hydrophilicity; Mechanical properties; Nanocomposites; Nanocrystals; Natural Materials; Organic Chemistry; Original Paper; Physical Chemistry; Plates (structural members); Polymer Sciences; Power law; Small angle X ray scattering; Sustainable Development; Thermogravimetric analysis; Toxicity; X-ray scattering
• ISSN: 0969-0239; 1572-882X
• DOI: 10.1007/s10570-017-1218-9

Collagen is an important biomaterial because it has many applications in the biomedical sector. However, the high hydrophilicity of collagen (COL) leads to easy swelling. Thus, controlling this property is highly desirable. In this work, cellulose nanocrystals (CNCs) dispersed in glycerol (GLI) were incorporated in the matrix collagen to tailor the hydrophilicity and mechanical properties. Study of the hydrophilicity of the bio-based nanocomposite was evaluated by contact angle measurement and thermogravimetric analysis. Mechanical analyses showed that CNCs are excellent reinforcing fillers to the collagen matrix. Synchrotron small-angle X-ray scattering was employed to investigate the nanostructures of COL/GLI/CNC nanocomposites and CNC water dispersion. CNC in concentrations up to 1 wt% presents an intermediate shape between a rod and a plane with a 9.34-nm radius of gyration ( R g ). Bio-based nanocomposites present two different structural levels with two types of particles with very different R g s. At the intermediate power-law regime, a large-scale mass fractal aggregate is observed. In the high-power-law regime, it is observed scattering from primary particles smaller than 1 nm. As the CNC concentration increases, the original particle distorts from a rod to a plate. The cytotoxicity assay indicates that the collagen and nanocomposites did not affect the cell viability of rat calvarial cells in vitro.