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Overexpression of Two Upstream Phospholipid Signaling Genes Improves Cold Stress Response and Hypoxia Tolerance, but Leads to Developmental Abnormalities in Barley
Phosphatidylinositol transfer protein (PITP) and phosphatidylinositol 4-kinase (PI4K) are very upstream regulatory elements of the phospholipid signaling pathway in the signal transduction network. Unlike in animal systems, their role in stress signaling is poorly understood in plants. To study this...
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Published in: | Plant molecular biology reporter 2019-08, Vol.37 (4), p.314-326 |
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creator | Gierczik, Krisztián Székely, András Ahres, Mohamed Marozsán-Tóth, Zsuzsa Vashegyi, Ildikó Harwood, Wendy Tóth, Balázs Galiba, Gábor Soltész, Alexandra Vágújfalvi, Attila |
description | Phosphatidylinositol transfer protein (PITP) and phosphatidylinositol 4-kinase (PI4K) are very upstream regulatory elements of the phospholipid signaling pathway in the signal transduction network. Unlike in animal systems, their role in stress signaling is poorly understood in plants. To study this area,
PITP
- and
PI4K
-overexpressing transgenic barley lines were developed. Morphological and developmental abnormalities were surveyed and characterized. It was revealed that the overexpression of the upstream signaling genes led to more phenotypic abnormalities than in other transgenic studies working with effector genes or even transcription factors. We hypothesize that this high level of abnormalities is the consequence of the modulation of the very upstream signal transduction pathway elements. On the other hand, we also revealed that overexpression of the
PITP
and
PI4K
genes increased stress tolerance during hypoxic cold stress, but not during salinity stress. Differences were also found in the level of frost tolerance between the transgenic overexpression plants and the recipient Golden Promise line. Molecular analysis showed that this improvement was not related to the most important cold responsive transcription factors, the
CBF
genes. We conclude that the transgenic method may be useful to prove the role of an upstream signaling element; however, due to the many developmental consequences that occur as side effects, it is a less advisable approach to achieve improved stress tolerance. |
doi_str_mv | 10.1007/s11105-019-01154-5 |
format | article |
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PITP
- and
PI4K
-overexpressing transgenic barley lines were developed. Morphological and developmental abnormalities were surveyed and characterized. It was revealed that the overexpression of the upstream signaling genes led to more phenotypic abnormalities than in other transgenic studies working with effector genes or even transcription factors. We hypothesize that this high level of abnormalities is the consequence of the modulation of the very upstream signal transduction pathway elements. On the other hand, we also revealed that overexpression of the
PITP
and
PI4K
genes increased stress tolerance during hypoxic cold stress, but not during salinity stress. Differences were also found in the level of frost tolerance between the transgenic overexpression plants and the recipient Golden Promise line. Molecular analysis showed that this improvement was not related to the most important cold responsive transcription factors, the
CBF
genes. We conclude that the transgenic method may be useful to prove the role of an upstream signaling element; however, due to the many developmental consequences that occur as side effects, it is a less advisable approach to achieve improved stress tolerance.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/s11105-019-01154-5</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>1-Phosphatidylinositol 4-kinase ; Abnormalities ; Barley ; Bioinformatics ; Biomedical and Life Sciences ; CBF protein ; Cold tolerance ; Genes ; Hypoxia ; Kinases ; Life Sciences ; Metabolomics ; Original Paper ; Phosphatidylinositol transfer protein ; Phospholipids ; Plant Breeding/Biotechnology ; Plant Sciences ; Proteomics ; Regulatory sequences ; Side effects ; Signal transduction ; Signaling ; Transcription factors ; Transgenic plants</subject><ispartof>Plant molecular biology reporter, 2019-08, Vol.37 (4), p.314-326</ispartof><rights>The Author(s) 2019</rights><rights>Plant Molecular Biology Reporter is a copyright of Springer, (2019). All Rights Reserved. © 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-e1fd52cde101603af973fc525d507ee04238eb35be05eac7a5c9b2ec8db8ad7a3</citedby><cites>FETCH-LOGICAL-c363t-e1fd52cde101603af973fc525d507ee04238eb35be05eac7a5c9b2ec8db8ad7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Gierczik, Krisztián</creatorcontrib><creatorcontrib>Székely, András</creatorcontrib><creatorcontrib>Ahres, Mohamed</creatorcontrib><creatorcontrib>Marozsán-Tóth, Zsuzsa</creatorcontrib><creatorcontrib>Vashegyi, Ildikó</creatorcontrib><creatorcontrib>Harwood, Wendy</creatorcontrib><creatorcontrib>Tóth, Balázs</creatorcontrib><creatorcontrib>Galiba, Gábor</creatorcontrib><creatorcontrib>Soltész, Alexandra</creatorcontrib><creatorcontrib>Vágújfalvi, Attila</creatorcontrib><title>Overexpression of Two Upstream Phospholipid Signaling Genes Improves Cold Stress Response and Hypoxia Tolerance, but Leads to Developmental Abnormalities in Barley</title><title>Plant molecular biology reporter</title><addtitle>Plant Mol Biol Rep</addtitle><description>Phosphatidylinositol transfer protein (PITP) and phosphatidylinositol 4-kinase (PI4K) are very upstream regulatory elements of the phospholipid signaling pathway in the signal transduction network. Unlike in animal systems, their role in stress signaling is poorly understood in plants. To study this area,
PITP
- and
PI4K
-overexpressing transgenic barley lines were developed. Morphological and developmental abnormalities were surveyed and characterized. It was revealed that the overexpression of the upstream signaling genes led to more phenotypic abnormalities than in other transgenic studies working with effector genes or even transcription factors. We hypothesize that this high level of abnormalities is the consequence of the modulation of the very upstream signal transduction pathway elements. On the other hand, we also revealed that overexpression of the
PITP
and
PI4K
genes increased stress tolerance during hypoxic cold stress, but not during salinity stress. Differences were also found in the level of frost tolerance between the transgenic overexpression plants and the recipient Golden Promise line. Molecular analysis showed that this improvement was not related to the most important cold responsive transcription factors, the
CBF
genes. We conclude that the transgenic method may be useful to prove the role of an upstream signaling element; however, due to the many developmental consequences that occur as side effects, it is a less advisable approach to achieve improved stress tolerance.</description><subject>1-Phosphatidylinositol 4-kinase</subject><subject>Abnormalities</subject><subject>Barley</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>CBF protein</subject><subject>Cold tolerance</subject><subject>Genes</subject><subject>Hypoxia</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Metabolomics</subject><subject>Original Paper</subject><subject>Phosphatidylinositol transfer protein</subject><subject>Phospholipids</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Sciences</subject><subject>Proteomics</subject><subject>Regulatory sequences</subject><subject>Side effects</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Transcription factors</subject><subject>Transgenic plants</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9UcFu2zAMFYoVaNbuB3YisOvcSlYU28cs69oCATq06VmQbTpRYEua6GTN9_RHqywDdtuBIAG-94jHx9hnwa8F58UNCSG4yrioUgk1zdQZmwhV5FlVivIDm_BCqqyaTfkF-0i05YnEy3LC3h73GPE1RCSy3oHvYPXbw0ugMaIZ4OfGU9j43gbbwrNdO9Nbt4Y7dEjwMITo92lY-D5tx6MIPCEF7wjBuBbuD8G_WgMr32M0rsGvUO9GWKJpCUYP33GPvQ8DutH0MK-dj0O6MNokah18M7HHwxU770xP-Olvv2QvP25Xi_ts-Xj3sJgvs0bO5Jih6FqVNy0KLmZcmq4qZNeoXLWKF4h8mssSa6lq5ApNUxjVVHWOTdnWpWkLIy_Zl5NucvVrhzTqrd_F5Jh0ns9EVaVXy4TKT6gmeqKInQ7RDiYetOD6GIY-haFTGPpPGFolkjyRKIHdGuM_6f-w3gH99ZDy</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Gierczik, Krisztián</creator><creator>Székely, András</creator><creator>Ahres, Mohamed</creator><creator>Marozsán-Tóth, Zsuzsa</creator><creator>Vashegyi, Ildikó</creator><creator>Harwood, Wendy</creator><creator>Tóth, Balázs</creator><creator>Galiba, Gábor</creator><creator>Soltész, Alexandra</creator><creator>Vágújfalvi, Attila</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20190801</creationdate><title>Overexpression of Two Upstream Phospholipid Signaling Genes Improves Cold Stress Response and Hypoxia Tolerance, but Leads to Developmental Abnormalities in Barley</title><author>Gierczik, Krisztián ; Székely, András ; Ahres, Mohamed ; Marozsán-Tóth, Zsuzsa ; Vashegyi, Ildikó ; Harwood, Wendy ; Tóth, Balázs ; Galiba, Gábor ; Soltész, Alexandra ; Vágújfalvi, Attila</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-e1fd52cde101603af973fc525d507ee04238eb35be05eac7a5c9b2ec8db8ad7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>1-Phosphatidylinositol 4-kinase</topic><topic>Abnormalities</topic><topic>Barley</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>CBF protein</topic><topic>Cold tolerance</topic><topic>Genes</topic><topic>Hypoxia</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>Metabolomics</topic><topic>Original Paper</topic><topic>Phosphatidylinositol transfer protein</topic><topic>Phospholipids</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Sciences</topic><topic>Proteomics</topic><topic>Regulatory sequences</topic><topic>Side effects</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>Transcription factors</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gierczik, Krisztián</creatorcontrib><creatorcontrib>Székely, András</creatorcontrib><creatorcontrib>Ahres, Mohamed</creatorcontrib><creatorcontrib>Marozsán-Tóth, Zsuzsa</creatorcontrib><creatorcontrib>Vashegyi, Ildikó</creatorcontrib><creatorcontrib>Harwood, Wendy</creatorcontrib><creatorcontrib>Tóth, Balázs</creatorcontrib><creatorcontrib>Galiba, Gábor</creatorcontrib><creatorcontrib>Soltész, Alexandra</creatorcontrib><creatorcontrib>Vágújfalvi, Attila</creatorcontrib><collection>SpringerOpen</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gierczik, Krisztián</au><au>Székely, András</au><au>Ahres, Mohamed</au><au>Marozsán-Tóth, Zsuzsa</au><au>Vashegyi, Ildikó</au><au>Harwood, Wendy</au><au>Tóth, Balázs</au><au>Galiba, Gábor</au><au>Soltész, Alexandra</au><au>Vágújfalvi, Attila</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of Two Upstream Phospholipid Signaling Genes Improves Cold Stress Response and Hypoxia Tolerance, but Leads to Developmental Abnormalities in Barley</atitle><jtitle>Plant molecular biology reporter</jtitle><stitle>Plant Mol Biol Rep</stitle><date>2019-08-01</date><risdate>2019</risdate><volume>37</volume><issue>4</issue><spage>314</spage><epage>326</epage><pages>314-326</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>Phosphatidylinositol transfer protein (PITP) and phosphatidylinositol 4-kinase (PI4K) are very upstream regulatory elements of the phospholipid signaling pathway in the signal transduction network. Unlike in animal systems, their role in stress signaling is poorly understood in plants. To study this area,
PITP
- and
PI4K
-overexpressing transgenic barley lines were developed. Morphological and developmental abnormalities were surveyed and characterized. It was revealed that the overexpression of the upstream signaling genes led to more phenotypic abnormalities than in other transgenic studies working with effector genes or even transcription factors. We hypothesize that this high level of abnormalities is the consequence of the modulation of the very upstream signal transduction pathway elements. On the other hand, we also revealed that overexpression of the
PITP
and
PI4K
genes increased stress tolerance during hypoxic cold stress, but not during salinity stress. Differences were also found in the level of frost tolerance between the transgenic overexpression plants and the recipient Golden Promise line. Molecular analysis showed that this improvement was not related to the most important cold responsive transcription factors, the
CBF
genes. We conclude that the transgenic method may be useful to prove the role of an upstream signaling element; however, due to the many developmental consequences that occur as side effects, it is a less advisable approach to achieve improved stress tolerance.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11105-019-01154-5</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 1-Phosphatidylinositol 4-kinase Abnormalities Barley Bioinformatics Biomedical and Life Sciences CBF protein Cold tolerance Genes Hypoxia Kinases Life Sciences Metabolomics Original Paper Phosphatidylinositol transfer protein Phospholipids Plant Breeding/Biotechnology Plant Sciences Proteomics Regulatory sequences Side effects Signal transduction Signaling Transcription factors Transgenic plants |
title | Overexpression of Two Upstream Phospholipid Signaling Genes Improves Cold Stress Response and Hypoxia Tolerance, but Leads to Developmental Abnormalities in Barley |
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