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Protein Polyacrylamide Gel Electrophoresis as a Common Vole Sibling Species Identification Method (Microtus arvalis Pallas, 1779 and M.rossiaemeridionalis Ognev, 1924 (Rodentia, Cricetidae))
A new protocol for the identification of two sibling species of voles Microtus arvalis and M . rossiaemeridionalis was proposed by electrophoresis of blood proteins in polyacrylamide gel (PAGE). Animals captured in ten districts of Moscow, Kaluga, and Samara oblasts and Moscow natural territories (1...
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| Published in: | Biology bulletin of the Russian Academy of Sciences 2019-07, Vol.46 (4), p.353-360 |
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| Main Authors: | , , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c316t-cf2fb83af6396dfc16624672010ee93add953b0351c12852e4a4c83c50429f5a3 |
| container_end_page | 360 |
| container_issue | 4 |
| container_start_page | 353 |
| container_title | Biology bulletin of the Russian Academy of Sciences |
| container_volume | 46 |
| creator | Zhigarev, I. A. Zhigarev, D. I. Alpatov, V. V. Lapkovsky, V. V. Malygin, V. M. Simak, S. V. |
| description | A new protocol for the identification of two sibling species of voles
Microtus arvalis
and
M
.
rossiaemeridionalis
was proposed by electrophoresis of blood proteins in polyacrylamide gel (PAGE). Animals captured in ten districts of Moscow, Kaluga, and Samara oblasts and Moscow natural territories (1030 individuals), as well as those taken from vivarium collections (five hybrids of the two species), were studied. A comparison of electrophoresis in PAGE, agarose gel, and cellulose acetate plates was carried out. The use of different organs and tissues for species identification was assessed. The relative electrophoretic mobility and the mass of a species-specific blood protein of the southern vole were determined. |
| doi_str_mv | 10.1134/S1062359019030154 |
| format | article |
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Microtus arvalis
and
M
.
rossiaemeridionalis
was proposed by electrophoresis of blood proteins in polyacrylamide gel (PAGE). Animals captured in ten districts of Moscow, Kaluga, and Samara oblasts and Moscow natural territories (1030 individuals), as well as those taken from vivarium collections (five hybrids of the two species), were studied. A comparison of electrophoresis in PAGE, agarose gel, and cellulose acetate plates was carried out. The use of different organs and tissues for species identification was assessed. The relative electrophoretic mobility and the mass of a species-specific blood protein of the southern vole were determined.</description><identifier>ISSN: 1062-3590</identifier><identifier>EISSN: 1608-3059</identifier><identifier>DOI: 10.1134/S1062359019030154</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Acetic acid ; Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; Cellulose acetate ; Ecology ; Electrophoretic mobility ; Gel electrophoresis ; Hybrids ; Life Sciences ; Microtus arvalis ; Microtus rossiaemeridionalis ; Sibling species ; Zoology</subject><ispartof>Biology bulletin of the Russian Academy of Sciences, 2019-07, Vol.46 (4), p.353-360</ispartof><rights>Pleiades Publishing, Inc. 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-cf2fb83af6396dfc16624672010ee93add953b0351c12852e4a4c83c50429f5a3</citedby><cites>FETCH-LOGICAL-c316t-cf2fb83af6396dfc16624672010ee93add953b0351c12852e4a4c83c50429f5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Zhigarev, I. A.</creatorcontrib><creatorcontrib>Zhigarev, D. I.</creatorcontrib><creatorcontrib>Alpatov, V. V.</creatorcontrib><creatorcontrib>Lapkovsky, V. V.</creatorcontrib><creatorcontrib>Malygin, V. M.</creatorcontrib><creatorcontrib>Simak, S. V.</creatorcontrib><title>Protein Polyacrylamide Gel Electrophoresis as a Common Vole Sibling Species Identification Method (Microtus arvalis Pallas, 1779 and M.rossiaemeridionalis Ognev, 1924 (Rodentia, Cricetidae))</title><title>Biology bulletin of the Russian Academy of Sciences</title><addtitle>Biol Bull Russ Acad Sci</addtitle><description>A new protocol for the identification of two sibling species of voles
Microtus arvalis
and
M
.
rossiaemeridionalis
was proposed by electrophoresis of blood proteins in polyacrylamide gel (PAGE). Animals captured in ten districts of Moscow, Kaluga, and Samara oblasts and Moscow natural territories (1030 individuals), as well as those taken from vivarium collections (five hybrids of the two species), were studied. A comparison of electrophoresis in PAGE, agarose gel, and cellulose acetate plates was carried out. The use of different organs and tissues for species identification was assessed. The relative electrophoretic mobility and the mass of a species-specific blood protein of the southern vole were determined.</description><subject>Acetic acid</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cellulose acetate</subject><subject>Ecology</subject><subject>Electrophoretic mobility</subject><subject>Gel electrophoresis</subject><subject>Hybrids</subject><subject>Life Sciences</subject><subject>Microtus arvalis</subject><subject>Microtus rossiaemeridionalis</subject><subject>Sibling species</subject><subject>Zoology</subject><issn>1062-3590</issn><issn>1608-3059</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kVtLMzEQhhdR8PgDvAt4o-BqTps2lx_FE1gsVr1dpslsjWQ3_ZKt0D_nbzO1ghciBCYwz_MmwxTFMaMXjAl5OWVUcVFpyjQVlFVyq9hjig5LQSu9ne-5Xa77u8V-Sm-UUikk3ys-JjH06DoyCX4FJq48tM4iuUFPrjyaPobFa4iYXCKQDxmFtg0deQkeydTNvOvmZLpA4zCRO4td7xpnoHeZGWP_Giw5HTuTH1lmO76Dz0ET8B7SOWGDgSbQWTK-iCElB9hidDa7X9jDvMP3TGkuyelj-AqHczKKzmDvLODZ2WGx04BPePRdD4rn66un0W15_3BzN_p3XxrBVF-ahjezoYBGCa1sY5hSXKoBp4wiagHW6krMqKiYYXxYcZQgzVCYikqumwrEQXGyyV3E8H-Jqa_fwjLmb6aaczVYr0DJTLENZdbjRGzqRXQtxFXNaL1m6l9ryg7fOCmz3RzjT_Lf0ieoCJSK</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Zhigarev, I. A.</creator><creator>Zhigarev, D. I.</creator><creator>Alpatov, V. V.</creator><creator>Lapkovsky, V. V.</creator><creator>Malygin, V. M.</creator><creator>Simak, S. V.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20190701</creationdate><title>Protein Polyacrylamide Gel Electrophoresis as a Common Vole Sibling Species Identification Method (Microtus arvalis Pallas, 1779 and M.rossiaemeridionalis Ognev, 1924 (Rodentia, Cricetidae))</title><author>Zhigarev, I. A. ; Zhigarev, D. I. ; Alpatov, V. V. ; Lapkovsky, V. V. ; Malygin, V. M. ; Simak, S. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-cf2fb83af6396dfc16624672010ee93add953b0351c12852e4a4c83c50429f5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acetic acid</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cellulose acetate</topic><topic>Ecology</topic><topic>Electrophoretic mobility</topic><topic>Gel electrophoresis</topic><topic>Hybrids</topic><topic>Life Sciences</topic><topic>Microtus arvalis</topic><topic>Microtus rossiaemeridionalis</topic><topic>Sibling species</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhigarev, I. A.</creatorcontrib><creatorcontrib>Zhigarev, D. I.</creatorcontrib><creatorcontrib>Alpatov, V. V.</creatorcontrib><creatorcontrib>Lapkovsky, V. V.</creatorcontrib><creatorcontrib>Malygin, V. M.</creatorcontrib><creatorcontrib>Simak, S. V.</creatorcontrib><collection>CrossRef</collection><jtitle>Biology bulletin of the Russian Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhigarev, I. A.</au><au>Zhigarev, D. I.</au><au>Alpatov, V. V.</au><au>Lapkovsky, V. V.</au><au>Malygin, V. M.</au><au>Simak, S. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein Polyacrylamide Gel Electrophoresis as a Common Vole Sibling Species Identification Method (Microtus arvalis Pallas, 1779 and M.rossiaemeridionalis Ognev, 1924 (Rodentia, Cricetidae))</atitle><jtitle>Biology bulletin of the Russian Academy of Sciences</jtitle><stitle>Biol Bull Russ Acad Sci</stitle><date>2019-07-01</date><risdate>2019</risdate><volume>46</volume><issue>4</issue><spage>353</spage><epage>360</epage><pages>353-360</pages><issn>1062-3590</issn><eissn>1608-3059</eissn><abstract>A new protocol for the identification of two sibling species of voles
Microtus arvalis
and
M
.
rossiaemeridionalis
was proposed by electrophoresis of blood proteins in polyacrylamide gel (PAGE). Animals captured in ten districts of Moscow, Kaluga, and Samara oblasts and Moscow natural territories (1030 individuals), as well as those taken from vivarium collections (five hybrids of the two species), were studied. A comparison of electrophoresis in PAGE, agarose gel, and cellulose acetate plates was carried out. The use of different organs and tissues for species identification was assessed. The relative electrophoretic mobility and the mass of a species-specific blood protein of the southern vole were determined.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1062359019030154</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 1062-3590 |
| ispartof | Biology bulletin of the Russian Academy of Sciences, 2019-07, Vol.46 (4), p.353-360 |
| issn | 1062-3590 1608-3059 |
| language | eng |
| recordid | cdi_proquest_journals_2267113464 |
| source | Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List |
| subjects | Acetic acid Biochemistry Biomedical and Life Sciences Cell Biology Cellulose acetate Ecology Electrophoretic mobility Gel electrophoresis Hybrids Life Sciences Microtus arvalis Microtus rossiaemeridionalis Sibling species Zoology |
| title | Protein Polyacrylamide Gel Electrophoresis as a Common Vole Sibling Species Identification Method (Microtus arvalis Pallas, 1779 and M.rossiaemeridionalis Ognev, 1924 (Rodentia, Cricetidae)) |
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