Use of Dibutyryl Cyclic Adenosine Monophosphate in Sperm Capacitation for in vitro Production of Bovine Embryos

The opportunity to create a chemically defined system for bovine sperm in vitro capacitation has been performed. 100 µM dibutyryl cyclic adenosine monophosphate (dbcAMP) used as a capacitating agent instead of heparin 10 µg/mL solution was administered in a Tyrode’s protein-free medium for fertiliza...

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Bibliographic Details
Published in:Biology bulletin of the Russian Academy of Sciences 2019-07, Vol.46 (4), p.327-331
Main Authors: Smetanina, I. G., Tatarinova, L. V., Krivokharchenko, A. S.
Format: Article
Language:English
Subjects:
Adenosine
Biochemistry
Biomedical and Life Sciences
Capacitation
Cell Biology
Cyclic AMP
Ecology
Embryos
Heparin
In vitro fertilization
Life Sciences
Oocytes
Proteins
Sperm
Zoology
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Summary:The opportunity to create a chemically defined system for bovine sperm in vitro capacitation has been performed. 100 µM dibutyryl cyclic adenosine monophosphate (dbcAMP) used as a capacitating agent instead of heparin 10 µg/mL solution was administered in a Tyrode’s protein-free medium for fertilization. In addition, the fertilization medium did not contain glucose or any sperm-activating additives. It has been proven for the first time that spermatozoa capacitated in the in vitro protein-free medium with the use of dbcAMP only are capable of highly efficient in vitro fertilization of the bovine oocytes, which were matured outside the body in a protein-free medium as well. Embryos developed to the 4- to 8-cell stage are chosen as criteria for normal fertilization.
ISSN:1062-3590
1608-3059
DOI:10.1134/S1062359019040137