Phyltetralin, 1,7,8‐trihydroxy 2‐naphtaldehyde, ethyl 8‐hydroxy‐8‐methyl‐tridecanoate and 1‐triacontanol from Phyllanthus amarus Schumach. & Thonn. inhibit phagocytic activity of human leucocytes

Objective To investigate the immunosuppressive effects of ethyl 8‐hydroxy‐8‐methyl‐tridecanoate, 1‐triacontanol, phyltetralin and 1,7,8‐trihydroxy 2‐naphtaldehyde isolated from Phyllanthus amarus Schumach. & Thonn. on chemotaxis, oxidative burst, CD18 complex expression and phagocytosis ability...

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Bibliographic Details
Published in:Journal of pharmacy and pharmacology 2019-09, Vol.71 (9), p.1451-1457
Main Authors: Yuandani,  , Jantan, Ibrahim, Husain, Khairana
Format: Article
Language:English
Subjects:
1,7,8‐trihydroxy 2‐naphtaldehyde
1‐triacontanol
Aspirin
Boyden chamber
CD18 antigen
Chemiluminescence
Chemotaxis
ethyl 8‐hydroxy‐8‐methyl‐tridecanoate
Flow cytometry
Immune system
immunosuppressive effects
Inhibition
Innate immunity
Leukocyte migration
Leukocytes (polymorphonuclear)
Neutrophils
Phagocytes
Phagocytosis
Phyllanthus amarus
Phyllanthus amarus Schumach. & Thonn
phyltetralin
Reactive oxygen species
Respiratory burst
Triacontanol
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Summary:Objective To investigate the immunosuppressive effects of ethyl 8‐hydroxy‐8‐methyl‐tridecanoate, 1‐triacontanol, phyltetralin and 1,7,8‐trihydroxy 2‐naphtaldehyde isolated from Phyllanthus amarus Schumach. & Thonn. on chemotaxis, oxidative burst, CD18 complex expression and phagocytosis ability of human leucocytes. Methods Polymorphonuclear neutrophil (PMN) migration activity was measured using a modified Boyden chamber, and inhibition on oxidative burst was determined by chemiluminescence assay. The effects on CD18 complex expression and phagocytosis ability were evaluated by flow cytometry. Key findings All compounds tested exhibited significant inhibitory effects on chemotaxis and respiratory burst. Among the compounds tested, phyltetralin demonstrated the highest inhibition on PMN chemotaxis with an IC50 value of 4.01 µm. Phyltetralin and 1,7,8‐trihydroxy 2‐naphtaldehyde showed strong inhibition against reactive oxygen species (ROS) production from PMA‐stimulated neutrophil with IC50 values of 0.73 and 0.79 µm, respectively, which were comparable to that of aspirin (0.77 µm). All compounds showed weak inhibition on CD18 expression and moderate inhibition on engulfment activity. Conclusion Compounds isolated from P. amarus possessed immunosuppressive effects on different lineages of innate immune system.
ISSN:0022-3573
2042-7158
DOI:10.1111/jphp.13139