Detection of Ralstonia solanacearum phylotype II, race 2 causing Moko disease and validation of genetic resistance observed in the hybrid plantain FHIA-21

Vascular wilt of banana and plantains, also known as Moko disease, is caused by Ralstonia solanacearum (Rs) phylotype II, and is the main bacterial disease affecting these crops in the Americas. Upon comparative sequence analysis of 44 Rs genomes we developed an improved PCR protocol based on the nu...

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Bibliographic Details
Published in:Tropical plant pathology 2019-08, Vol.44 (4), p.371-379
Main Authors: Pardo, Juan M., López-Alvarez, Diana, Ceballos, German, Alvarez, Elizabeth, Cuellar, Wilmer J.
Format: Article
Language:English
Subjects:
Bacterial diseases
Biomedical and Life Sciences
Breeding
Genomes
Genotypes
Inoculation
Life Sciences
Musaceae
Nucleotide sequence
Nucleotides
Plant Pathology
Plantains
Ralstonia solanacearum
Roots
Short Communication
Signs and symptoms
Wilt
Wounds
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Summary:Vascular wilt of banana and plantains, also known as Moko disease, is caused by Ralstonia solanacearum (Rs) phylotype II, and is the main bacterial disease affecting these crops in the Americas. Upon comparative sequence analysis of 44 Rs genomes we developed an improved PCR protocol based on the nucleotide sequence of a gene coding for a hypothetical DUF3313 domain protein. Next, we tested the detection protocol with two Rs inoculation methods to validate field resistance reported in the hybrid plantain genotype FHIA-21, previously identified as susceptible to Moko disease in greenhouse experiments in which wounds were caused to the roots prior to inoculation. By using an inoculation method without causing wounds to the roots, we confirmed resistance of FHIA-21 to Moko disease (no Rs was detected by PCR in inoculated plants). In contrast, the field-susceptible genotype Dominico Hartón developed severe symptoms of Moko disease, regardless of the inoculation method used. FHIA-21 showed an area under the disease progress curve (AUDPC) close to zero, while Dominico Hartón plants showed AUDPC values ranging from 65.8 to 88.4. The availability and analysis of genomic data facilitates the development of improved pathogen detection tools that together with the availability of improved inoculation methods and tolerant genotypes to Moko disease will be of great use in Musa breeding programs.
ISSN:1983-2052
1982-5676
1983-2052
DOI:10.1007/s40858-019-00282-3