Molecular identification of biological contaminants in different drinking water resources of the Jazan region, Saudi Arabia

• Published in: Journal of water and health 2019-08, Vol.17 (4), p.622-632
• Main Authors: Abada, Emad, Al-Fifi, Zarraq, Al-Rajab, Abdul Jabbar, Mahdhi, Mosbah, Sharma, Mukul
• Format: Article
• Language: English
• Subjects: Antibiotics; Bacteria; Bottled water; Cefotaxime; Chemical contaminants; Clavulanic acid; Contaminants; Contamination; Culture media; Culture techniques; Deoxyribonucleic acid; DNA; Drinking Water; E coli; Erythromycin; Fluorides; Groundwater; Humans; Methods; Nucleic acids; Nucleotide sequence; Pathogens; PCR; Penicillin; Physicochemical analysis; Polymerase chain reaction; Public health; Purification; Rifampin; RNA; rRNA 16S; Salmonella; Saudi Arabia; Sulfamethoxazole; Supplies; Trimethoprim; Water analysis; Water Microbiology; Water pollution; Water purification; Water Quality; Water quality standards; Water Resources; Water sampling
• ISSN: 1477-8920; 1996-7829
• DOI: 10.2166/wh.2019.019

Drinking water quality plays a remarkable role in human infections and diseases. This study used polymerase chain reaction (PCR) techniques to detect bacterial pathogens. In addition, a physicochemical analysis was performed on drinking water samples from several sources. A total of 123 drinking water samples were collected from different areas in the Jazan region in Saudi Arabia: ground water (40 samples), bottled water (15 samples), tap water (52 samples), and water purification shops (16 samples). To isolate the bacterial pathogens, the water samples were spread on Nutrient and MacConkey agar media, and the grown pathogens were then identified by the 16S ribosomal RNA technique. In 87 (70.7%) of the 123 drinking water samples, there was no pathogen growth on the two-culture medium. However, 36 (29.3%) of the samples were found to be contaminated with bacteria. The physicochemical analysis indicated that the water samples were within the Saudi drinking water standards. The bacteria were resistant to Cefotaxime, Cefotaxime/Clavulanic acid, Erythromycin, Penicillin G, Rifampin and Sulfamethoxazole-Trimethoprim, respectively. The findings suggest that in Jazan, bottled water is a safer source of potable water than tap water. The contamination in the water may be occurring at the reservoirs rather than the water sources.