Highly polymorphic microsatellite loci for the Parsley frog (Pelodytes punctatus): characterization and testing for cross-species amplification

A microsatellite library was developed using genomic DNA of the Parsley frog, Pelodytes punctatus, an amphibian species which inhabits Mediterranean temporary pond systems. Number of alleles and heterozygosity ranged from 10 to 25 and from 0.66 to 0.90, respectively. Cross-species amplification was...

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Bibliographic Details
Published in:Conservation genetics 2009-06, Vol.10 (3), p.665-668
Main Authors: van de Vliet, Mirjam S, Diekmann, Onno E, Serrão, Ester T. A, Beja, Pedro
Format: Article
Language:English
Subjects:
Amphibian conservation genetics
Amphibians
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation biology
Conservation Biology/Ecology
Cross-species amplification
Ecology
Evolutionary Biology
Frogs
Gene loci
Genetic diversity
Life Sciences
microsatellite repeats
Parsley frog
Pelodytes punctatus
Plant Genetics and Genomics
Polymorphism
Population genetics
Technical Note
Temporary ponds
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Summary:A microsatellite library was developed using genomic DNA of the Parsley frog, Pelodytes punctatus, an amphibian species which inhabits Mediterranean temporary pond systems. Number of alleles and heterozygosity ranged from 10 to 25 and from 0.66 to 0.90, respectively. Cross-species amplification was successful for 13 of the 15 developed loci for the related species, Pelodytes ibericus. The high levels of polymorphism revealed by these loci will be extremely useful for characterizing the population genetic diversity and structure and to estimate levels of dispersal and gene flow in the species P. punctatus and P. ibericus.
ISSN:1566-0621
1572-9737
DOI:10.1007/s10592-008-9609-y