Isolation and characterization of ten new polymorphic microsatellite loci in the mud crab, Scylla paramamosain

We developed polymerase chain primers for ten microsatellite loci in the mud crab, Scylla paramamosain . All markers were obtained from a (CA) 15 and (CT) 15 -enrichment DNA library, and characterized in 30 individuals from one wild population. The number of alleles per locus varies between 8 and 18...

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Bibliographic Details
Published in:Conservation genetics 2009-12, Vol.10 (6), p.1877-1878
Main Authors: Xu, Xiao-Jun, Wang, Gui-Zhong, Wang, Ke-Jian, Li, Shao-Jing
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation
Conservation Biology/Ecology
Crustaceans
Ecology
Evolutionary Biology
Genetic markers
Life Sciences
Plant Genetics and Genomics
Population genetics
Technical Note
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Summary:We developed polymerase chain primers for ten microsatellite loci in the mud crab, Scylla paramamosain . All markers were obtained from a (CA) 15 and (CT) 15 -enrichment DNA library, and characterized in 30 individuals from one wild population. The number of alleles per locus varies between 8 and 18, and the observed and expected heterozygosities ranged from 0.6207 to 0.9333 and from 0.5886 to 0.9243, respectively. These polymorphic loci provide a valuable tool for population genetic analysis and parentage determination in this species.
ISSN:1566-0621
1572-9737
DOI:10.1007/s10592-009-9843-y