Tetranucleotide markers from the loggerhead sea turtle (Caretta caretta) and their cross-amplification in other marine turtle species

The loggerhead sea turtle (Caretta caretta) is a federally threatened species and listed as endangered by the World Conservation Union (IUCN). We describe primers and polymerase chain reaction (PCR) conditions to amplify 11 novel tetranucleotide microsatellite loci from the loggerhead sea turtle. We...

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Bibliographic Details
Published in:Conservation genetics 2009-06, Vol.10 (3), p.577-580
Main Authors: Shamblin, Brian M, Faircloth, Brant C, Dodd, Mark G, Bagley, Dean A, Ehrhart, Llewellyn M, Dutton, Peter H, Frey, Amy, Nairn, Campbell J
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Aquatic reptiles
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Endangered & extinct species
Endangered species
Evolutionary Biology
Gene loci
Genetic markers
Life Sciences
Plant Genetics and Genomics
Reptiles & amphibians
Technical Note
Threatened species
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Summary:The loggerhead sea turtle (Caretta caretta) is a federally threatened species and listed as endangered by the World Conservation Union (IUCN). We describe primers and polymerase chain reaction (PCR) conditions to amplify 11 novel tetranucleotide microsatellite loci from the loggerhead sea turtle. We tested primers using samples from 22 females that nested at Melbourne Beach, Florida (USA). Primer pairs yielded an average of 11.2 alleles per locus (range of 4-24), an average observed heterozygosity of 0.83 (range 0.59-0.96), and an average polymorphic information content of 0.80 (range 0.62-0.94). We also demonstrate the utility of these primers, in addition to primers for 15 loci previously described, for amplifying microsatellite loci in four additional species representing the two extant marine turtle families: olive ridley (Lepidochelys olivacea), hawksbill (Eretmochelys imbricata), green turtle (Chelonia mydas), and leatherback (Dermochelys coriacea).
ISSN:1566-0621
1572-9737
DOI:10.1007/s10592-008-9573-6