Genetic study on Bacillus cereus

• Published in: Research journal of pharmacy and technology 2019-03, Vol.12 (3), p.1301-1306
• Main Author: Hadi, Huda W.
• Format: Article
• Language: English
• Subjects: Antibiotics; Bacteria; Curing; Deoxyribonucleic acid; DNA; Drug resistance; E coli; Mutagenesis; Plasmids; Soil contamination
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.5958/0974-360X.2019.00218.X

B. cereus contain one or two Plasmids which can encode for Bacteriocin and tetracyclin resistance[9]. [...]current study aims to detect chromate resistance gene or genes in B. cereus bacteria that isolated from soil, and try proof their location, whether it are on the plasmid itself or on the chromosome. The high resistance of chromate in these bacteria, some papers return it to aggregate chromate molecules in plasma membrane which lead to detoxification of chromate [22]whereas others suggest resistance to chromate wasn't related to the plasma membrane, but it related only to the soluble protein molecules also [23] or by enzymatic action that works in aerobic and anaerobic conditions which reduce chromate from quaternary to ternary chromate [24]. Results from curing study in this study indicates preliminary for the probability exists of chromate resistance genes or gene on the plasmid rather than on a chromosome. that's consistent with the information we received from [30] suggested that the bacteria B. sp. becomes sensitive to heavy metals after curing the plasmid ,and the same thing happened with the spices B. cereus which lost the chromate character [31]. Sidorkina O, Saparbaev M and Laval J. Effects of nitrous acid treatment on the survival and mutagenesis of Escherichia coli cells lacking base excision repair (hypoxanthine-DNA glycosylase-ALK A protein) and/or nucleotide excision repair.