Secretion of the Caulobacter crescentus S-layer protein: Further localization of the C-terminal secretion signal and its use for secretion of recombinant patterns

The secretion signal of the Caulobacter crescentus S-layer protein (RsaA) was localized to the C-terminal 82 amino acids of the molecule. Protein yield studies showed that 336 or 242 C-terminal residues of RsaA mediated secretion of greater than 50 mg of a cellulase passenger protein per liter to th...

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Bibliographic Details
Published in:Journal of bacteriology 2000-06, Vol.182 (11), p.3298
Main Authors: Bingle, Wade H, Nomellini, John F, Smit, John
Format: Article
Language:English
Subjects:
Amino acids
Bacteriology
Proteins
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Summary:The secretion signal of the Caulobacter crescentus S-layer protein (RsaA) was localized to the C-terminal 82 amino acids of the molecule. Protein yield studies showed that 336 or 242 C-terminal residues of RsaA mediated secretion of greater than 50 mg of a cellulase passenger protein per liter to the culture fluids.
ISSN:0021-9193
1098-5530