PhaQ, a new class of poly-beta-hydroxybutyrate (PHB)-responsive repressor, reguilates phaQ and phaP (phasin) expression in Bacillus megaterium through interaction with PHB

Bacillus megaterium can produce poly-Beta-hydroxybutyrate (PHB) as carbon and energy storage materials. We now report that the phaQ gene, which is located upstream of the phasin-encoding phaP gene, codes for a new class of transcriptional regulator that negatively controls expression of both phaQ an...

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Bibliographic Details
Published in:Journal of bacteriology 2004-05, Vol.186 (10), p.3015
Main Authors: Tian-Ren, Lee, Lin, Jer-Sheng, Shih-Shin, Wang, Shaw, Gwo-Chyuan
Format: Article
Language:English
Subjects:
Bacteriology
Energy
Gene expression
Online Access:Get full text
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Summary:Bacillus megaterium can produce poly-Beta-hydroxybutyrate (PHB) as carbon and energy storage materials. We now report that the phaQ gene, which is located upstream of the phasin-encoding phaP gene, codes for a new class of transcriptional regulator that negatively controls expression of both phaQ and phaP. A PhaQ binding site that plays a role in this control has been identified by gel mobility shift assays and DNase I footprinting analysis. We have also provided evidence that PhaQ could sense the presence of PHB in vivo and that artificial PHB granules could inhibit the formation of PhaQ-DNA complex in vitro by binding to PhaQ directly. These suggest that PhaQ is a PHB-responsive repressor. [PUBLICATION ABSTRACT]
ISSN:0021-9193
1098-5530