A Vitamin B^sub 12^-Based System for Conditional Expression Reveals dksA To Be an Essential Gene in Myxococcus xanthus

Myxococcus xanthus is a prokaryotic model system for the study of multicellular development and the response to blue light. The previous analyses of these processes and the characterization of new genes would benefit from a robust system for controlled gene expression, which has been elusive so far...

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Bibliographic Details
Published in:Journal of bacteriology 2009-05, Vol.191 (9), p.3108
Main Authors: García-Moreno, Diana, Polanco, María Carmen, Navarro-Avilés, Gloria, Murillo, Francisco J, Padmanabhan, S, Elías-Arnanz, Montserrat
Format: Article
Language:English
Subjects:
Amino acids
Bacteria
Bacteriology
Gene expression
Genes
Ribonucleic acid
RNA
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Summary:Myxococcus xanthus is a prokaryotic model system for the study of multicellular development and the response to blue light. The previous analyses of these processes and the characterization of new genes would benefit from a robust system for controlled gene expression, which has been elusive so far for this bacterium. Here, we describe a system for conditional expression of genes in M. xanthus based on our recent finding that vitamin B... and CarH, a MerR-type transcriptional repressor, together downregulate a photoinducible promoter. Using this system, we confirmed that M. xanthus rpoN, encoding ..., is an essential gene, as reported earlier. We then tested it with ftsZ and dksA. In most bacteria, ftsZ is vital due to its role in cell division, whereas null mutants of dksA, whose product regulates the stringent response via transcriptional control of rRNA and amino acid biosynthesis promoters, are viable but cause pleiotropic effects. As with rpoN, it was impossible to delete endogenous ftsZ or dksA in M. xanthus except in a merodiploid background carrying another functional copy, which indicates that these are essential genes. B...-based conditional expression of ftsZ was insufficient to provide the high intracellular FtsZ levels required. With dksA, as with rpoN, cells were viable under permissive but not restrictive conditions, and depletion of DksA or ... produced filamentous, aberrantly dividing cells. dksA thus joins rpoN in a growing list of genes dispensable in many bacteria but essential in M. xanthus. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0021-9193
1098-5530