Regulation of Spess in Streptococcus pyogenes by pH and NaCl: a Model for In Vivo Gene Expression

For a pathogen such as Streptococcus pyogenes, ecological success is determined by its ability to sense the environment and mount an appropriate adaptive transcriptional response. Thus, determining conditions for analyses of gene expression in vitro that are representative of the in vivo environment...

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Bibliographic Details
Published in:Journal of bacteriology 2006-01, Vol.188 (2), p.399
Main Authors: Loughman, Jennifer A, Caparon, Michael
Format: Article
Language:English
Subjects:
Bacteriology
Cell culture
Gene expression
Molecular biology
Pathogens
Signal transduction
Online Access:Get full text
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Summary:For a pathogen such as Streptococcus pyogenes, ecological success is determined by its ability to sense the environment and mount an appropriate adaptive transcriptional response. Thus, determining conditions for analyses of gene expression in vitro that are representative of the in vivo environment is critical for understanding the contributions of transcriptional response pathways to pathogenesis. In this study, we determined that the gene encoding the SpeB cysteine protease is up-regulated over the course of infection in a murine soft-tissue model. Conditions were identified, including growth phase, acidic pH, and an NaCl concentration of
ISSN:0021-9193
1098-5530