Phytochemical Screening on Champaka pushpam ( Michelia champaca )

• Published in: Research journal of pharmacy and technology 2019-07, Vol.12 (7), p.3541-3546
• Main Authors: Shejale, Savita R., Yeligar, Veerendra. C.
• Format: Article
• Language: English
• Subjects: Amino acids; Carbohydrates; Flavonoids; Flowers & plants; Fruits; Herbal medicine; Hydrocarbons; Leaves; Phytochemicals; Solvents; Sterols
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.5958/0974-360X.2019.00603.6

The use of herbal medicines for the treatment of diseases is safe and traditional. [...]medicinal plants have been receiving great attention because of their safety. Michelia champaca L. is an evergreen or semideciduous, graceful, small to medium sized tree up to 50 m tall, bole straight, cylindrical, up to 200 cm in diameter, without buttresses, bark surface smooth, grey to greyish-white, inner bark fibrous, yellow to brown, crown conical to cylindrical, branchlet sterete with annular scars of caducous stipules, glabrous, apical bud covered by sericeous lanceolate stipules, leaves are simple, entire, arranged spirally, lamina ellipticlanceolate, apex acuminate with twisted acumen, base acute to attenuate, margins slightly undulate, glabrous, chartaceous, midrib nearly flat above, secondary nerves 12-16 pairs, stipules adnate to or free from the petiole, flowers on short, axillary brachyblast, solitary or rarely in pairs, large, tepals 6-21, in 3-6 usually subequal whorls, pale-yellow with strong ordour, stamens many, anthers with a short to prominently elongated connective, gynoecium stipitate with spirally arranged, free or connate carpels containing many ovules, fruiting carpels dehiscing along the dorsal suture when free or fused and forming a fleshy or woody syncarp, seed hanging from its funicle, brown and 1-4 in fruit. First determine free sugar content of the extract. Test for Anthraquinone glycoside: a) Borntragers test for anthraquinone Glycoside: To 3 ml extract, add dilute sulphuric acid boil and filter, to cold filtrate add equal volume benzene or chloroform shake well separate organic solvent, add ammonia, ammonical layers shows pinkish red colour. b) Modified borntragers test for C-glycoside: To 5 ml extract add 5 ml 5 % ferric chloride solution and 5 ml dilute HCL, heat for 5 minute in boiling water bath.