Complexity of dissolved organic matter in the molecular size dimension: insights from coupled size exclusion chromatography electrospray ionisation mass spectrometry

This paper investigates the relationship between apparent size distribution and molecular complexity of dissolved organic matter from the natural environment. We used a high pressure size exclusion chromatography (HPSEC) method coupled to UV-Vis diode array detection (UV-DAD) and electrospray ionisa...

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Bibliographic Details
Published in:Faraday discussions 2019-08, Vol.218, p.52-71
Main Authors: Hawkes, J. A, Sjöberg, P. J. R, Bergquist, J, Tranvik, L. J
Format: Article
Language:English
Subjects:
Chromatography
Complexity
Coupling (molecular)
Dissolved organic matter
Electrospraying
Ionization
Ions
Low molecular weights
Mass spectrometry
Molecular weight
Scientific imaging
Size distribution
Size exclusion chromatography
Spectroscopy
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Summary:This paper investigates the relationship between apparent size distribution and molecular complexity of dissolved organic matter from the natural environment. We used a high pressure size exclusion chromatography (HPSEC) method coupled to UV-Vis diode array detection (UV-DAD) and electrospray ionisation mass spectrometry (ESI-MS) in order to compare the apparent size of natural organic matter, determined by HPSEC-UV and the molecular mass determined online by ESI-MS. We found that there was a clear discrepancy between the two methods, and found evidence for an important pool of organic matter that has a strong UV absorbance and no ESI-MS signal. Contrary to some previous research, we found no evidence that apparently high molecular weight organic matter is constituted by aggregates of low molecular weight (
ISSN:1359-6640
1364-5498
1364-5498
DOI:10.1039/c8fd00222c