Extended depth-of-field microscopic imaging using a polynomial transmissive phase plate

•A microscope with a polynomial phase plate (PPP) is presented.•A USAF resolution test target was tested with and without the PPP.•An in-vitro pig’s retina was tested with and without the PPP.•Excellent results obtained within the extended depth which is around 60 µm. In this paper, a reflective lig...

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Bibliographic Details
Published in:Measurement : journal of the International Measurement Confederation 2019-10, Vol.145, p.765-768
Main Author: Ibrahim, Dahi Ghareab Abdelsalam
Format: Article
Language:English
Subjects:
Depth of field
Image processing systems
Image reconstruction-restoration
Lens system design
Medical devices
Medical electronics
Microscopes
Optical properties
Optical transfer functions
Phase plates
Phase transitions
Polynomials
Retina
Three-dimensional microscopy
White light
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Summary:•A microscope with a polynomial phase plate (PPP) is presented.•A USAF resolution test target was tested with and without the PPP.•An in-vitro pig’s retina was tested with and without the PPP.•Excellent results obtained within the extended depth which is around 60 µm. In this paper, a reflective light microscope for extending the depth-of-field with a polynomial phase plate is presented. The microscope with and without the polynomial phase plate is tested on imaging a USAF resolution test target and an in-vitro pig’s retina. Experimental results show that the polynomial phase plate can recover volumetric images identical to in-focus images within an acceptable range. We claim that the polynomial phase plate can be used in vertical scanning white-light interference microscopes and medical devices to improve the measurements of thick samples in real-time.
ISSN:0263-2241
1873-412X
DOI:10.1016/j.measurement.2019.06.011