Evaluation of the mastascanelite image analysis system for measuring zones of inhibition in disc diffusion susceptibility tests

In this evaluation a mastascanelite image analysis system is compared with manual measurement of disc diffusion inhibition zones. Data for 213 diverse organisms and a total of 1679 organism/antibiotic combinations gave an overall correlation coefficient of 0.988. The mean difference between readings...

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Bibliographic Details
Published in:British journal of biomedical science 2003-01, Vol.60 (3), p.133-135
Main Authors: Clarke, R.E.H., Winstanley, T.G., Ridgway, E.J.
Format: Article
Language:English
Subjects:
Bacteria - drug effects
computer-assisted
Disc diffusion
Humans
Image processing
Image Processing, Computer-Assisted - methods
Microbial Sensitivity Tests - instrumentation
Microbial Sensitivity Tests - methods
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Summary:In this evaluation a mastascanelite image analysis system is compared with manual measurement of disc diffusion inhibition zones. Data for 213 diverse organisms and a total of 1679 organism/antibiotic combinations gave an overall correlation coefficient of 0.988. The mean difference between readings was +0.425 mm, with 95% confidence limits of ±2.94 mm, and the majority of scanned zones (97.51%) fell within ±3 mm of the manual measurement. The mastascanelite system forms part of a laboratory suite and was found to be objective, accurate and rapid, reading and interpreting each plate in less than a second. Interfacing to the laboratory computer system facilitated data handling and performance control.
ISSN:0967-4845
2474-0896
DOI:10.1080/09674845.2003.11783688