ELECTROSPUN MATERIALS FOR TENDON AND MUSCLE ENGINEERING: TOWARDS THE RECONSTRUCTION OF THE MYOTENDINOUS JUNCTION

Bioengineering of elements of the musculo-skeletal system is rich in studies concerning bone reconstruction. Among them, the culture of cells on a osteogenic electrospun scaffold under dynamic conditions is an interesting option. However, one should also consider the reconstruction of tendon and mus...

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Bibliographic Details
Published in:International journal of artificial organs 2019-08, Vol.42 (8)
Main Authors: Beldjilali-Labro, M, Garcia, A Garcia, Farhat, F, Dermigny, Q, Jellali, R, Bedoui, F, Dufresne, M, Legallais, C, Grosset, J-F
Format: Article
Language:English
Subjects:
Alignment
Bioengineering
Biomedical materials
Bone marrow
Cell culture
Cell differentiation
Cell fusion
Collagen
Differentiation
Electrical stimuli
Electrospinning
Mimicry
Muscles
Myotubes
Nanofibers
Phenotypes
Reconstruction
Scaffolds
Skeletal system
Stem cells
Stretching
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Summary:Bioengineering of elements of the musculo-skeletal system is rich in studies concerning bone reconstruction. Among them, the culture of cells on a osteogenic electrospun scaffold under dynamic conditions is an interesting option. However, one should also consider the reconstruction of tendon and muscle finally mimicking a continuum from bone to muscle. Poly-caprolactone (PCL) 10% was electrospun on a rotating collector whose velocity varied to get random or aligned nanofibers. Bone marrow stem cells (BMSCs) and C2C12 cells were respectively seeded on the materials. Mechanical or electrical stresses were applied to the biohybrid scaffolds to differentiate cells towards tendon's or muscle's phenotypes, in the absence any specific biochemical factors. After 2 weeks of culture under dynamic stretching (1Hz, 4% strain for an hour followed by 11h of rest), BMSCs were found to align in the stretching direction, presented increased levels of tenomodulin and neosynthetized collagen, compared to static conditions. C2C12 differentiation until aligned myotubes stage was more difficult to achieve. We proposed to add a PEG microstructure to guide cell growth. Combined to electrical stimulation, it led to the cells fusion into very long myotubes hosting many nuclei, as expected. In this study, we managed to grow two different types of cells and generate their differentiation towards tendon and muscle lineage, in the absence of any differentiation factors. The next step is to implement the co-culture on different areas of the same electrospun scaffold, to let the cells proliferate and study the events occurring at the junction.
ISSN:0391-3988
1724-6040