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Genetic diversity and growth promoting characteristics of diazotrophic bacteria isolated from 20 genotypes of Brachiaria spp
Background and aims Diazotrophic bacteria, including those of the genus Azospirillum and Nitrospirillum , colonize Brachiaria genotypes and contribute to plant development through nitrogen fixation, production of phytohormones and bioavailability of nutrients. This study aimed to determine the phylo...
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| Published in: | Plant and soil 2020-06, Vol.451 (1-2), p.187-205 |
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| container_issue | 1-2 |
| container_start_page | 187 |
| container_title | Plant and soil |
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| creator | Ribeiro, Nathália Virgínia da Silva Vidal, Márcia Soares Barrios, Sanzio Carvalho Lima Baldani, Vera Lúcia Divan Baldani, José Ivo |
| description | Background and aims
Diazotrophic bacteria, including those of the genus
Azospirillum
and
Nitrospirillum
, colonize
Brachiaria
genotypes and contribute to plant development through nitrogen fixation, production of phytohormones and bioavailability of nutrients. This study aimed to determine the phylogenetic positioning and evaluate the functional abilities of diazotrophic bacteria isolated from
Brachiaria
genotypes.
Methods
Diazotrophic bacterial counting and isolation were carried out with rhizosphere soil and root samples from 20
Brachiaria
genotypes after inoculation in nitrogen-free semi-solid NFb and LGI media. The isolates were analyzed using 16S rRNA and
nif
H sequences, and tested for their functional abilities to produce auxin and siderophores, to solubilize phosphate and zinc, and to degrade cellulose.
Results
The diazotrophic population ranged from 10
2
to 10
8
g
−1
rhizosphere soil or roots. Sequencing of 16S rRNA from 213 isolates confirmed the presence of genera
Azospirillum
and
Nitrospirillum
, and revealed the presence of 14 other diazotrophic genera. The genus
Nitrospirillum
was detected colonizing all niches of most
Brachiaria
species. A PCA analysis showed a positive correlation between the ability to produce siderophores with the ability to produce IAA; and between phosphate and zinc solubilisation.
Conclusions
The results showed a high diversity of diazotrophic bacterial species colonizing 20
Brachiaria
genotypes and revealed the presence of bacteria with variable growth-promoting characteristics, highlighting their potential as good candidates for the development of biofertilizers. |
| doi_str_mv | 10.1007/s11104-019-04263-y |
| format | article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2280220030</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A629341463</galeid><sourcerecordid>A629341463</sourcerecordid><originalsourceid>FETCH-LOGICAL-c273y-6846521192a2f47561d3118ea9611cf14b5387c9ff72d8a2d4f95041054f815c3</originalsourceid><addsrcrecordid>eNp9kUFrHCEUxyW0kE2aL5CTkPNs3lPHmTmmS5sGAr20kJsYR2cNu-NU3ZYJ_fBxdwq5FQ-i_n5Pn39CrhHWCNDcJkQEUQF2FQgmeTWfkRXWDa9q4PIDWQFwVkHTPZ2Ti5Re4LhGuSJ_7-1osze0979tTD7PVI89HWL4k7d0imEfsh8HarY6apNt9KnQiQZXDP0acgzTtujPy6GmPoWdzranrriUAR3sGPI82ZPzuRTZen0E0zR9Ih-d3iV79W--JD-_fvmx-VY9fr9_2Nw9VoY1fK5kK2TNEDummRNNLbHniK3VnUQ0DsVzzdvGdM41rG8164XrahAItXAt1oZfkpulbunn18GmrF7CIY7lSsVYC4yV34BCrRdq0Dur_OhKc9qU0du9N2G0zpf9O8k6LlBIXgS2CCaGlKJ1aop-r-OsENQxFrXEokos6hSLmovEFykVeBxsfH_Lf6w3I6OROg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2280220030</pqid></control><display><type>article</type><title>Genetic diversity and growth promoting characteristics of diazotrophic bacteria isolated from 20 genotypes of Brachiaria spp</title><source>Springer Link</source><source>JSTOR Archival Journals</source><creator>Ribeiro, Nathália Virgínia da Silva ; Vidal, Márcia Soares ; Barrios, Sanzio Carvalho Lima ; Baldani, Vera Lúcia Divan ; Baldani, José Ivo</creator><creatorcontrib>Ribeiro, Nathália Virgínia da Silva ; Vidal, Márcia Soares ; Barrios, Sanzio Carvalho Lima ; Baldani, Vera Lúcia Divan ; Baldani, José Ivo</creatorcontrib><description>Background and aims
Diazotrophic bacteria, including those of the genus
Azospirillum
and
Nitrospirillum
, colonize
Brachiaria
genotypes and contribute to plant development through nitrogen fixation, production of phytohormones and bioavailability of nutrients. This study aimed to determine the phylogenetic positioning and evaluate the functional abilities of diazotrophic bacteria isolated from
Brachiaria
genotypes.
Methods
Diazotrophic bacterial counting and isolation were carried out with rhizosphere soil and root samples from 20
Brachiaria
genotypes after inoculation in nitrogen-free semi-solid NFb and LGI media. The isolates were analyzed using 16S rRNA and
nif
H sequences, and tested for their functional abilities to produce auxin and siderophores, to solubilize phosphate and zinc, and to degrade cellulose.
Results
The diazotrophic population ranged from 10
2
to 10
8
g
−1
rhizosphere soil or roots. Sequencing of 16S rRNA from 213 isolates confirmed the presence of genera
Azospirillum
and
Nitrospirillum
, and revealed the presence of 14 other diazotrophic genera. The genus
Nitrospirillum
was detected colonizing all niches of most
Brachiaria
species. A PCA analysis showed a positive correlation between the ability to produce siderophores with the ability to produce IAA; and between phosphate and zinc solubilisation.
Conclusions
The results showed a high diversity of diazotrophic bacterial species colonizing 20
Brachiaria
genotypes and revealed the presence of bacteria with variable growth-promoting characteristics, highlighting their potential as good candidates for the development of biofertilizers.</description><identifier>ISSN: 0032-079X</identifier><identifier>EISSN: 1573-5036</identifier><identifier>DOI: 10.1007/s11104-019-04263-y</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Analysis ; Bacteria ; Bacterial genetics ; Bioavailability ; Biodiversity ; Biofertilizers ; Biological diversity ; Biomedical and Life Sciences ; Brachiaria ; Cellulose ; Correlation analysis ; Ecology ; Fertilizers ; Fixation ; Genetic diversity ; Genetic research ; Genotype ; Genotypes ; Gibberellins ; Growth ; Inoculation ; Life Sciences ; NifH gene ; Nitrogen ; Nitrogen fixation ; Nitrogenation ; Nutrients ; Phosphates ; Phylogeny ; Plant hormones ; Plant Physiology ; Plant Sciences ; Polysaccharides ; Regular Article ; Rhizosphere ; RNA ; rRNA 16S ; Semisolids ; Siderophores ; Soil Science & Conservation ; Soils ; Species diversity ; Zinc</subject><ispartof>Plant and soil, 2020-06, Vol.451 (1-2), p.187-205</ispartof><rights>Springer Nature Switzerland AG 2019</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer Nature Switzerland AG 2019.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c273y-6846521192a2f47561d3118ea9611cf14b5387c9ff72d8a2d4f95041054f815c3</citedby><cites>FETCH-LOGICAL-c273y-6846521192a2f47561d3118ea9611cf14b5387c9ff72d8a2d4f95041054f815c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Ribeiro, Nathália Virgínia da Silva</creatorcontrib><creatorcontrib>Vidal, Márcia Soares</creatorcontrib><creatorcontrib>Barrios, Sanzio Carvalho Lima</creatorcontrib><creatorcontrib>Baldani, Vera Lúcia Divan</creatorcontrib><creatorcontrib>Baldani, José Ivo</creatorcontrib><title>Genetic diversity and growth promoting characteristics of diazotrophic bacteria isolated from 20 genotypes of Brachiaria spp</title><title>Plant and soil</title><addtitle>Plant Soil</addtitle><description>Background and aims
Diazotrophic bacteria, including those of the genus
Azospirillum
and
Nitrospirillum
, colonize
Brachiaria
genotypes and contribute to plant development through nitrogen fixation, production of phytohormones and bioavailability of nutrients. This study aimed to determine the phylogenetic positioning and evaluate the functional abilities of diazotrophic bacteria isolated from
Brachiaria
genotypes.
Methods
Diazotrophic bacterial counting and isolation were carried out with rhizosphere soil and root samples from 20
Brachiaria
genotypes after inoculation in nitrogen-free semi-solid NFb and LGI media. The isolates were analyzed using 16S rRNA and
nif
H sequences, and tested for their functional abilities to produce auxin and siderophores, to solubilize phosphate and zinc, and to degrade cellulose.
Results
The diazotrophic population ranged from 10
2
to 10
8
g
−1
rhizosphere soil or roots. Sequencing of 16S rRNA from 213 isolates confirmed the presence of genera
Azospirillum
and
Nitrospirillum
, and revealed the presence of 14 other diazotrophic genera. The genus
Nitrospirillum
was detected colonizing all niches of most
Brachiaria
species. A PCA analysis showed a positive correlation between the ability to produce siderophores with the ability to produce IAA; and between phosphate and zinc solubilisation.
Conclusions
The results showed a high diversity of diazotrophic bacterial species colonizing 20
Brachiaria
genotypes and revealed the presence of bacteria with variable growth-promoting characteristics, highlighting their potential as good candidates for the development of biofertilizers.</description><subject>Analysis</subject><subject>Bacteria</subject><subject>Bacterial genetics</subject><subject>Bioavailability</subject><subject>Biodiversity</subject><subject>Biofertilizers</subject><subject>Biological diversity</subject><subject>Biomedical and Life Sciences</subject><subject>Brachiaria</subject><subject>Cellulose</subject><subject>Correlation analysis</subject><subject>Ecology</subject><subject>Fertilizers</subject><subject>Fixation</subject><subject>Genetic diversity</subject><subject>Genetic research</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Gibberellins</subject><subject>Growth</subject><subject>Inoculation</subject><subject>Life Sciences</subject><subject>NifH gene</subject><subject>Nitrogen</subject><subject>Nitrogen fixation</subject><subject>Nitrogenation</subject><subject>Nutrients</subject><subject>Phosphates</subject><subject>Phylogeny</subject><subject>Plant hormones</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polysaccharides</subject><subject>Regular Article</subject><subject>Rhizosphere</subject><subject>RNA</subject><subject>rRNA 16S</subject><subject>Semisolids</subject><subject>Siderophores</subject><subject>Soil Science & Conservation</subject><subject>Soils</subject><subject>Species diversity</subject><subject>Zinc</subject><issn>0032-079X</issn><issn>1573-5036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kUFrHCEUxyW0kE2aL5CTkPNs3lPHmTmmS5sGAr20kJsYR2cNu-NU3ZYJ_fBxdwq5FQ-i_n5Pn39CrhHWCNDcJkQEUQF2FQgmeTWfkRXWDa9q4PIDWQFwVkHTPZ2Ti5Re4LhGuSJ_7-1osze0979tTD7PVI89HWL4k7d0imEfsh8HarY6apNt9KnQiQZXDP0acgzTtujPy6GmPoWdzranrriUAR3sGPI82ZPzuRTZen0E0zR9Ih-d3iV79W--JD-_fvmx-VY9fr9_2Nw9VoY1fK5kK2TNEDummRNNLbHniK3VnUQ0DsVzzdvGdM41rG8164XrahAItXAt1oZfkpulbunn18GmrF7CIY7lSsVYC4yV34BCrRdq0Dur_OhKc9qU0du9N2G0zpf9O8k6LlBIXgS2CCaGlKJ1aop-r-OsENQxFrXEokos6hSLmovEFykVeBxsfH_Lf6w3I6OROg</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Ribeiro, Nathália Virgínia da Silva</creator><creator>Vidal, Márcia Soares</creator><creator>Barrios, Sanzio Carvalho Lima</creator><creator>Baldani, Vera Lúcia Divan</creator><creator>Baldani, José Ivo</creator><general>Springer International Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7ST</scope><scope>7T7</scope><scope>7X2</scope><scope>88A</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>SOI</scope></search><sort><creationdate>20200601</creationdate><title>Genetic diversity and growth promoting characteristics of diazotrophic bacteria isolated from 20 genotypes of Brachiaria spp</title><author>Ribeiro, Nathália Virgínia da Silva ; Vidal, Márcia Soares ; Barrios, Sanzio Carvalho Lima ; Baldani, Vera Lúcia Divan ; Baldani, José Ivo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c273y-6846521192a2f47561d3118ea9611cf14b5387c9ff72d8a2d4f95041054f815c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analysis</topic><topic>Bacteria</topic><topic>Bacterial genetics</topic><topic>Bioavailability</topic><topic>Biodiversity</topic><topic>Biofertilizers</topic><topic>Biological diversity</topic><topic>Biomedical and Life Sciences</topic><topic>Brachiaria</topic><topic>Cellulose</topic><topic>Correlation analysis</topic><topic>Ecology</topic><topic>Fertilizers</topic><topic>Fixation</topic><topic>Genetic diversity</topic><topic>Genetic research</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Gibberellins</topic><topic>Growth</topic><topic>Inoculation</topic><topic>Life Sciences</topic><topic>NifH gene</topic><topic>Nitrogen</topic><topic>Nitrogen fixation</topic><topic>Nitrogenation</topic><topic>Nutrients</topic><topic>Phosphates</topic><topic>Phylogeny</topic><topic>Plant hormones</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polysaccharides</topic><topic>Regular Article</topic><topic>Rhizosphere</topic><topic>RNA</topic><topic>rRNA 16S</topic><topic>Semisolids</topic><topic>Siderophores</topic><topic>Soil Science & Conservation</topic><topic>Soils</topic><topic>Species diversity</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ribeiro, Nathália Virgínia da Silva</creatorcontrib><creatorcontrib>Vidal, Márcia Soares</creatorcontrib><creatorcontrib>Barrios, Sanzio Carvalho Lima</creatorcontrib><creatorcontrib>Baldani, Vera Lúcia Divan</creatorcontrib><creatorcontrib>Baldani, José Ivo</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Plant and soil</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ribeiro, Nathália Virgínia da Silva</au><au>Vidal, Márcia Soares</au><au>Barrios, Sanzio Carvalho Lima</au><au>Baldani, Vera Lúcia Divan</au><au>Baldani, José Ivo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity and growth promoting characteristics of diazotrophic bacteria isolated from 20 genotypes of Brachiaria spp</atitle><jtitle>Plant and soil</jtitle><stitle>Plant Soil</stitle><date>2020-06-01</date><risdate>2020</risdate><volume>451</volume><issue>1-2</issue><spage>187</spage><epage>205</epage><pages>187-205</pages><issn>0032-079X</issn><eissn>1573-5036</eissn><abstract>Background and aims
Diazotrophic bacteria, including those of the genus
Azospirillum
and
Nitrospirillum
, colonize
Brachiaria
genotypes and contribute to plant development through nitrogen fixation, production of phytohormones and bioavailability of nutrients. This study aimed to determine the phylogenetic positioning and evaluate the functional abilities of diazotrophic bacteria isolated from
Brachiaria
genotypes.
Methods
Diazotrophic bacterial counting and isolation were carried out with rhizosphere soil and root samples from 20
Brachiaria
genotypes after inoculation in nitrogen-free semi-solid NFb and LGI media. The isolates were analyzed using 16S rRNA and
nif
H sequences, and tested for their functional abilities to produce auxin and siderophores, to solubilize phosphate and zinc, and to degrade cellulose.
Results
The diazotrophic population ranged from 10
2
to 10
8
g
−1
rhizosphere soil or roots. Sequencing of 16S rRNA from 213 isolates confirmed the presence of genera
Azospirillum
and
Nitrospirillum
, and revealed the presence of 14 other diazotrophic genera. The genus
Nitrospirillum
was detected colonizing all niches of most
Brachiaria
species. A PCA analysis showed a positive correlation between the ability to produce siderophores with the ability to produce IAA; and between phosphate and zinc solubilisation.
Conclusions
The results showed a high diversity of diazotrophic bacterial species colonizing 20
Brachiaria
genotypes and revealed the presence of bacteria with variable growth-promoting characteristics, highlighting their potential as good candidates for the development of biofertilizers.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s11104-019-04263-y</doi><tpages>19</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-079X |
| ispartof | Plant and soil, 2020-06, Vol.451 (1-2), p.187-205 |
| issn | 0032-079X 1573-5036 |
| language | eng |
| recordid | cdi_proquest_journals_2280220030 |
| source | Springer Link; JSTOR Archival Journals |
| subjects | Analysis Bacteria Bacterial genetics Bioavailability Biodiversity Biofertilizers Biological diversity Biomedical and Life Sciences Brachiaria Cellulose Correlation analysis Ecology Fertilizers Fixation Genetic diversity Genetic research Genotype Genotypes Gibberellins Growth Inoculation Life Sciences NifH gene Nitrogen Nitrogen fixation Nitrogenation Nutrients Phosphates Phylogeny Plant hormones Plant Physiology Plant Sciences Polysaccharides Regular Article Rhizosphere RNA rRNA 16S Semisolids Siderophores Soil Science & Conservation Soils Species diversity Zinc |
| title | Genetic diversity and growth promoting characteristics of diazotrophic bacteria isolated from 20 genotypes of Brachiaria spp |
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