Delimitation of sympatric Palaemon (Decapoda, Palaemonidae) species of the Laguna Madre, Mexico

• Published in: Zoologica scripta 2019-09, Vol.48 (5), p.667-678
• Main Authors: Jordán‐Hernández, María, Rodríguez‐Almaráz, Gabino, Favela‐Lara, Susana
• Format: Article
• Language: English
• Subjects: Animal appendages; Aquatic crustaceans; Brackishwater crustaceans; Crustaceans; Diagnostic systems; Gene sequencing; Interspecific; Laguna Madre; Marine crustaceans; Meristic counts; Mitochondria; mitochondrial DNA; Morphology; Morphometry; Multivariate analysis; Palaemon; Palaemonetes pugio; Phenotypic plasticity; Phylogeny; Physical characteristics; Plastic properties; Plasticity; Principal components analysis; Rostrum; Shrimps; Similarity; Species; Sympatric populations; sympatric species; taxonomic delimitation; Taxonomy; Telson; Validity
• ISSN: 0300-3256; 1463-6409
• DOI: 10.1111/zsc.12364

Grass shrimps Palaemon mundusnovus, P. pugio and P. vulgaris sympatrically inhabit in the Laguna Madre, Mexico. They exhibit very close morphological similarity and overlap in their diagnostic characteristics, which has hindered certainty in their identification and has raised doubt regarding their taxonomic validity. In this work, we analyse intra‐ and interspecific morphometric and meristic variability through a multivariate analysis, and we determine the molecular variation using mitochondrial sequences of the 12S, 16S and COX1 genes to confirm the validity of the three taxa as having distinct lineages or to recognize a smaller number of species with phenotypic plasticity. Our results corroborate the taxonomic validity of the three species; however, there is intraspecific plasticity, interspecific overlap of characteristics and greater morphological and molecular similarity between the species P. mundusnovus and P. pugio, whereas P. vulgaris was better delimited. These species form a monophyletic group but the phylogenetic relationship obtained is discussed. Telson length was the primary variable in the principal components analysis, whereas the length of the propodus of the second pereiopod was the best discriminant. The range of variation reported in the characteristics linked to the rostrum is extended, and its influence on the separation of these species is recognized. The joint application of multivariate analysis from morphological variables and molecular tools is recommended to clarify the taxonomic status of species featuring close morphological similarity and sympatric distribution.