Helichrysum italicum G. Don subsp. italicum from Herzegovina: Volatile Composition, Variations during Seasons, Total Polyphenols, Acetylcholinesterase Inhibition and Antioxidant Activity

The chemical composition of essential oils isolated from immortelle (Helicrysum italicum subsp. italicum) collected in Herzegovina during five different periods, was investigated by GC/MS analysis. The main compounds were [alpha]-pinene (15.7%) and [gamma]-curcumene (12.8%), followed by 4,6,9-trimet...

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Bibliographic Details
Published in:Croatica chemica acta 2019-01, Vol.92 (1), p.69-77
Main Authors: Talic, Stanislava, Odak, Ilijana, Bevanda, Anita Ma, Crnjac, Nikolina, Pastar, Mate
Format: Article
Language:English
Subjects:
Analysis
Antioxidants
Antioxidants (Nutrients)
Chemical activity
Chemical composition
Chromatography
Essential oils
Food
Inhibition
Oils & fats
Organic chemistry
Phenols (Class of compounds)
Polyphenols
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Summary:The chemical composition of essential oils isolated from immortelle (Helicrysum italicum subsp. italicum) collected in Herzegovina during five different periods, was investigated by GC/MS analysis. The main compounds were [alpha]-pinene (15.7%) and [gamma]-curcumene (12.8%), followed by 4,6,9-trimethyldec-8-en-3,5-dione (8.7%), neryl acetate (6.9%), limonene (6.4%) and [beta]-selinene (5.3%). In total, 69 components were identified whose share changed over the vegetative cycle. Antioxidant activity of methanolic extracts of immortelle were determined according to DPPH ([IC.sub.50] = 23-34 [micro]g/mL) and FRAP (29 [micro]g/mL is equivalent to 1.1-2.2 mM [Fe.sup.2+]) methods. Acetylcholinesterase inhibitory potential, investigated by modified Ellman's assay and determined as [IC.sub.50] values, were 340-440 [micro]g/mL for methanol extracts and 135 [micro]g/mL for essential oil. Metanolic extracts showed strong antioxidant activity and potential to inhibit AChE. Essential oil possesses complex chemical composition, inhibition activity of AChE and weak antioxidant capacity. Keywords: Helichrysum italicum subsp. italicum, Asteraceae, essential oil composition, [alpha]-pinene, [gamma]-curcumene, antioxidant activity.
ISSN:0011-1643
1334-417X
DOI:10.5562/cca3475