High throughput functional screening of an ion channel library for drug safety and efficacy

Development of a large, representative library of ion channel-expressing cell lines is described. Validation on a full range of automated patch clamp and fluorescence illumination platforms is ongoing. Library “books” can be mixed and matched into channel panels according to tissue, therapeutic area...

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Bibliographic Details
Published in:European biophysics journal 2009-03, Vol.38 (3), p.273-278
Main Author: Brown, Arthur M
Format: Article
Language:English
Subjects:
Animals
Automated fluorescence
Automated patch clamp
Automation
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Cardiac channel panel[trade mark sign]
Cardiology
Cell Biology
Cell Line
Cricetinae
Cricetulus
Drug Evaluation, Preclinical - methods
Drug-Related Side Effects and Adverse Reactions
ERG1 Potassium Channel
Ether-A-Go-Go Potassium Channels - antagonists & inhibitors
Ether-A-Go-Go Potassium Channels - biosynthesis
Ether-A-Go-Go Potassium Channels - physiology
High throughput screening
Humans
Ion Channel Gating - drug effects
Ion channel library
Ion Channels - biosynthesis
Ion Channels - genetics
Ion Channels - physiology
Ions
Life Sciences
Membrane Biology
Myocardium - metabolism
Nanotechnology
Neurobiology
Patch-Clamp Techniques
Pharmacology
Review
Risk assessment
Toxicology
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Description
Summary:Development of a large, representative library of ion channel-expressing cell lines is described. Validation on a full range of automated patch clamp and fluorescence illumination platforms is ongoing. Library “books” can be mixed and matched into channel panels according to tissue, therapeutic area and ion channel family. Unexpected results using a cardiac channel panel show that this panel may serve as a biomarker for cardiac risk assessment.
ISSN:0175-7571
1432-1017
DOI:10.1007/s00249-008-0356-2