Formation of and changes in phytohormone levels in response to stress during the manufacturing process of oolong tea (Camellia sinensis)

• Published in: Postharvest biology and technology 2019-11, Vol.157, p.110974, Article 110974
• Main Authors: Zeng, Lanting, Wang, Xuewen, Liao, Yinyin, Gu, Dachuan, Dong, Fang, Yang, Ziyin
• Format: Article
• Language: English
• Subjects: Abscisic acid; Aroma; Biosynthesis; Camellia sinensis; Dehydration; Gene expression; Jasmonic acid; Manufacturing; Manufacturing industry; Oolong tea; Phytohormones; Plant hormones; Plucking; Signal processing; Stresses; Tea; Volatile; Volatiles; Withering; Wounding
• ISSN: 0925-5214; 1873-2356
• DOI: 10.1016/j.postharvbio.2019.110974

•Jasmonic acid and abscisic acid increased during oolong tea manufacturing process.•Wounding stress quickly increased jasmonic acid levels in tea leaves.•Continuous wounding stress slowed down jasmonic acid decrease in tea leaves.•CsAOS2 played an important role in jasmonic acid synthesis under wounding stress.•Dehydration stress enhanced synthesis and signaling transduction of abscisic acid. As important upstream signals, phytohormones regulate the plant volatiles’ biosynthesis under various stresses. The formation of some characteristic aromas during the manufacturing process of oolong tea (postharvest stage) is due to the defense responses of tea leaves to stress. This study investigates the formation of phytohormone in response to stresses during the manufacturing process of oolong tea. Jasmonic acid (JA) and abscisic acid (ABA) levels enhanced during the manufacturing processes (enzyme-active stage) of oolong tea. Wounding from plucking activated JA synthetic gene expression, resulting in increased levels of JA (p ≤  0.01), and continuous wounding from the turn over stage further enhanced JA synthesis (p ≤  0.05). Dehydration stress during the withering stage activated ABA synthetic gene expression resulting in an increase of ABA (p ≤  0.01). The study advances the understanding of key upstream signals, JA and ABA, during the manufacturing process of oolong tea.