Supramolecular off-on-off fluorescent biosensor for total Free thyroid hormones detection based on their differential binding with cucurbit[7]uril to fluorescent perylene derivative

• Published in: Journal of photochemistry and photobiology. A, Chemistry. Chemistry., 2019-09, Vol.382, p.111945, Article 111945
• Main Authors: El-Sheshtawy, Hamdy S., El-Refaey, Ahmed, El-Khouly, Mohamed E.
• Format: Article
• Language: English
• Subjects: Binding; Biosensor; Biosensors; Cucurbituril; Fluorescent indicators; Hormones; Host-guest; Hyperthyroidism; Menopause; Perylene; Thyroid; Thyroid gland; Thyroid hormones; Thyroxine; Total thyroid hormones; Triiodothyronine
• ISSN: 1010-6030; 1873-2666
• DOI: 10.1016/j.jphotochem.2019.111945

[Display omitted] •A novel host-guest biosensor composed of perylene and cucurbit[7]uril is established for determine the total thyroid hormones.•The assembled biosensor takes place through the competitive binding of hormones with CB7 to the fluorescent perylene dye.•The assembled biosensor showed a detection limit of 16.10 mM (for triiodothyronine T3) and 5.66 mM (for thyroxine T4).•The proposed sensor accurately determines the total thyroid hormones (T3 and T4), which is crucial for the hyperthyroidism clinical diagnosis.•This simple strategy is economical and tackle tedious and longtime procedures for other methods. A novel host-guest biosensor composed of perylene dye derivatives and cucurbit[7]uril [CB7] is established for the determination of total thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The sensor was based on the competitive binding of the hormones (T3 and T4) with cucurbit[7]uril [CB7] to the fluorescent perylene derivative, N,N′-di(2-(trimethylammoniumiodide)ethylene) perylenediimide (TAIPDI). The binding constants between T3 and T4 with TAIPDI were determined to be 3.34 × 104 and 1.7 × 105 M–1, respectively, while the binding between TAIPDI and CB7 was found to be 1.48 × 103 M−1. The assembled biosensor showed different signal between T3 and T4 in the range of 0–106 and 0–25 μM and detection limit of 16.1 and 5.66 μM, respectively. The proposed sensor not only differentiates between the T3 and T4 hormones, but also accurately determines the total thyroid hormones, which is crucial for the hyperthyroidism clinical diagnosis. This strategy based on the use of fluorescent probe combined with macrocycle host is economical and tackle tedious and longtime procedures for similar methods.