Transdermal delivery of small interfering RNAs with topically applied mesoporous silica nanoparticles for facile skin cancer treatment

Small interfering RNA (siRNA) is a promising tool for the treatment of skin disorders including skin squamous cell carcinoma (SCC). This article develops a topical formulation for the transdermal delivery of siRNA. The formulation is built on mesoporous silica nanoparticles (MSNPs) with a loading ca...

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Bibliographic Details
Published in:Nanoscale 2019-09, Vol.11 (36), p.1741-1751
Main Authors: Lio, Daniel Chin Shiuan, Liu, Chenghao, Oo, Myo Myo Swe, Wiraja, Christian, Teo, Melissa Hui Yen, Zheng, Mengjia, Chew, Sharon Wan Ting, Wang, Xiaomeng, Xu, Chenjie
Format: Article
Language:English
Subjects:
Administration, Cutaneous
Animals
Biomarkers
Cancer
Carcinoma, Squamous Cell - drug therapy
Carcinoma, Squamous Cell - metabolism
Carcinoma, Squamous Cell - pathology
Drug Delivery Systems
Humans
Mice
Mice, SCID
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - therapeutic use
Oligonucleotides - chemistry
Oligonucleotides - pharmacology
Porosity
RNA, Small Interfering - chemistry
RNA, Small Interfering - pharmacology
Silicon dioxide
Silicon Dioxide - chemistry
Silicon Dioxide - pharmacology
Skin Neoplasms - drug therapy
Skin Neoplasms - metabolism
Skin Neoplasms - pathology
Xenograft Model Antitumor Assays
Xenotransplantation
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Summary:Small interfering RNA (siRNA) is a promising tool for the treatment of skin disorders including skin squamous cell carcinoma (SCC). This article develops a topical formulation for the transdermal delivery of siRNA. The formulation is built on mesoporous silica nanoparticles (MSNPs) with a loading capacity of 1.4 μg of oligonucleotide per mg of MSNPs. Cell experiments are employed to study the functionality of the formulation including the cellular uptake, the qualitative and quantitative detection of specific gene biomarkers. The clinical potential of this system is examined by topically delivering siRNA targeting TGFβR-1 (TGFβR-1) to the SCC in a mouse xenograft model. In comparison to the controls, MSNPs containing TGFβR-1 siRNA show a 2-fold suppression of TGFβR-1. Development of siRNA-loaded mesoporous Silica nanoparticles coated with poly- l -lysine for enhanced transdermal drug delivery in skin cancer treatment.
ISSN:2040-3364
2040-3372
DOI:10.1039/c9nr06303j