Recurrent somatic embryogenesis and plant regeneration from seedlings of Hepatica nobilis Schreb

A method for secondary somatic embryogenesis was developed on embryos derived from embryogenic callus formed on Hepatica nobilis seedlings. Somatic embryogenesis (SE) was induced on seedlings (on the hypocotyl and epicotyl parts) grown on the Murashige and Skoog (1962) medium (MS) supplemented with...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2015-03, Vol.120 (3), p.1203-1207
Main Authors: Szewczyk-Taranek, Bożena, Pawłowska, Bożena
Format: Article
Language:English
Subjects:
Acclimatization
Anemone (plant)
Benzyladenine
Biomedical and Life Sciences
Callus
callus formation
Cytokinins
Embryonic growth stage
Embryos
Germination
greenhouses
Growth regulators
heart
Hepatica nobilis
hypocotyls
Life Sciences
Naphthaleneacetic acid
organ culture
Plant Genetics and Genomics
Plant growth
Plant Pathology
Plant Physiology
Plant Sciences
Plantlets
Regeneration
Research Note
Seedlings
Somatic embryogenesis
somatic embryos
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Summary:A method for secondary somatic embryogenesis was developed on embryos derived from embryogenic callus formed on Hepatica nobilis seedlings. Somatic embryogenesis (SE) was induced on seedlings (on the hypocotyl and epicotyl parts) grown on the Murashige and Skoog (1962) medium (MS) supplemented with 1 µM naphthaleneacetic acid (NAA), and/or 0.1 µM 6-benzyladenine (BA) and on medium without plant growth regulators (PGR). The best response of embryogenic callus formation was observed on the medium containing 1 µM NAA alone or with 0.1 µM BA. Individual somatic embryos, formed on embryogenic callus on the medium without PGR (MS0), at heart, torpedo and cotyledonary stage, were transferred to the media where secondary somatic embryo formation and development into plantlets occurred. Although the most efficient repetitive cycles of secondary SE were recorded for all stages of somatic embryos (heart, torpedo, cotyledonary) on the MS0 medium (77.8–87.4 %), secondary somatic embryos were also obtained on all media supplemented with cytokinins. The best rate of somatic embryos germination was achieved on MS media with 0.2 µM NAA and 2 µM BA, and 0.1 µM NAA and 1 µM BA (48.8–52.0 %) when more mature embryos (cotyledonary stage) were used. Plantlets grown from somatic embryos were successfully acclimatized to greenhouse conditions.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-014-0661-7