Photoelectrochemical platform for glucose sensing based on g-C3N4/ZnIn2S4 composites coupled with bi-enzyme cascade catalytic in-situ precipitation

• Published in: Sensors and actuators. B, Chemical Chemical, 2019-10, Vol.297, p.126818, Article 126818
• Main Authors: Zhang, Xing Yue, Liu, Shi Gang, Zhang, Wen Jie, Wang, Xiao Hu, Han, Lei, Ling, Yu, Li, Nian Bing, Luo, Hong Qun
• Format: Article
• Language: English
• Subjects: Bi-enzyme cascade; Biosensors; Carbon nitride; Catalysis; Electrodes; Enzymes; g-C3N4/ZnIn2S4 composites; Glucose; Glucose oxidase; Hydrogen peroxide; in-situ precipitation; Naphthol; Oxidation; Particulate composites; Peroxidase; Photoelectric effect; Photoelectric emission; Photoelectrochemical biosensor; Signal processing
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2019.126818

[Display omitted] •A novel bi-enzyme photoelectrochemical sensor was constructed to detect glucose.•The g-C3N4/ZnIn2S4 composites with better photoactivity were used in PEC sensing.•This strategy can reduce interference of reducing species in biological samples.•The strategy owned good selectivity and sensitivity for detection of glucose. A unique bi-enzyme photoelectrochemical (PEC) biosensor is developed for selective and sensitive detection of glucose based on bi-enzyme cascade catalytic in-situ precipitation for signal amplification. In this design, the composites of ZnIn2S4 and graphitic carbon nitride (g-C3N4/ZnIn2S4) with better photoelectrochemical properties are used as the photoactive material. Subsequently, horseradish peroxidase (HRP) and glucose oxidase (GOx) are anchored on the modified electrode surface. In the presence of 4-chloro-1-naphthol (4-CN) and glucose, GOx catalyzes the oxidation of glucose to produce H2O2, which oxidizes 4-CN to generate a precipitate in the presence of HRP. The precipitate on the electrode surface can dramatically reduce the photocurrent signal. In the light of the signal magnification process, the bi-enzyme biosensor presents a “signal-off” sensing mode for glucose detection, which displays a dynamic detection range of 1–10000 μM with a low limit of detection of 0.28 μM. Furthermore, the constructed PEC bi-enzyme biosensor exhibits a tremendous potential for quantitative detection of glucose in practical applications.