Lysosome-targetable red-emitting ratiometric fluorescent probe for hypobromous acid imaging in living cells

• Published in: Sensors and actuators. B, Chemical Chemical, 2019-10, Vol.297, p.126826, Article 126826
• Main Authors: Qu, Wangbo, Li, Kaibin, Han, Deman, Zhong, Xinxin, Chen, Caixia, Liang, Xiuxia, Liu, Heng
• Format: Article
• Language: English
• Subjects: Cells (biology); Fluorescence imaging; Fluorescent indicators; Hypobromous acid; Ratiometric fluorescent probe; Red-emitting; Selectivity; Toxicity
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2019.126826

[Display omitted] •A highly selective 1,8-naphthalimide-based red-emitting ratiometric fluorescent probe Lyso-NpSN has been developed.•Probe Lyso-NpSN showed fast response to HOBr within 12 s.•Lyso-NpSN possessed excellent lysosomal targetable ability in living cells.•Lyso-NpSN could be successfully applied for ratiometrically imaging exogenous and endogenous HOBr in living cells. As one of the most important reactive oxygen species, hypobromous acid (HOBr) has been reported to be linked to a large body of diseases. Although some limited of HOBr-selective probes have been designed, there was no probes reported capable of sensing of HOBr with lysosome-targetable ability and red-emitting ratiometric fluorescence. Herein, we presented a 1,8-naphthalimide-based fluorescent probe Lyso-NpSN for rapid detection of HOBr over a wide pH range with high selectivity over other reactive oxygen species and sensitivity, for the first time. Furthermore, the probe was found to be low cytotoxicity to cells and had enabled to visualize exogenous and endogenous HOBr in living cells.