Peroxide generation by p47^sup phox^-Src activation of Nox2 has a key role in protein kinase C-induced arterial smooth muscle contraction

Protein kinase C (PKC) stimulation of NAD(P)H oxidases (Nox) is an important component of multiple vascular disease processes; however, the relationship between oxidase activation and the regulation of vascular smooth muscle contraction by PKC remains poorly understood. Therefore, we examined the si...

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Bibliographic Details
Published in:American journal of physiology. Heart and circulatory physiology 2009-04, Vol.296 (4), p.H1048
Main Authors: Gupte, Sachin A, Kaminski, Pawel M, George, Shimran, Kouznestova, Lioubov, Olson, Susan C, Mathew, Rajamma, Hintze, Thomas H, Wolin, Michael S
Format: Article
Language:English
Subjects:
Coronary vessels
Kinases
Muscular system
Rodents
Vein & artery diseases
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Summary:Protein kinase C (PKC) stimulation of NAD(P)H oxidases (Nox) is an important component of multiple vascular disease processes; however, the relationship between oxidase activation and the regulation of vascular smooth muscle contraction by PKC remains poorly understood. Therefore, we examined the signaling cascade of PKC-elicited Nox activation and the role of superoxide and hydrogen peroxide in mediating PKC-induced vascular contraction. Endothelium-denuded bovine coronary arteries showed a PKC-dependent basal production of lucigenin (5 ...M)-detected Nox oxidase-derived superoxide, which was stimulated fourfold by PKC activation with 10 ...M phorbol 12,13-dibutyrate (PDBu). PDBu appeared to increase superoxide generation by Nox2 through both p47... and peroxide-dependent Src activation mechanisms based on the actions of inhibitors, properties of Src phosphorylation, and the loss of responses in aorta from mice deficient in Nox2 and p47... The actions of inhibitors of contractile regulating mechanisms, scavengers of superoxide and peroxide, and responses in knockout mouse aortas suggest that a major component of the contraction elicited by PDBu appeared to be mediated through peroxide derived from Nox2 activation stimulating force generation through Rho kinase and calmodulin kinase-II mechanisms. Superoxide generated by PDBu also attenuated relaxation to nitroglycerin. Peroxide-derived from Nox2 activation by PKC appeared to be a major contributor to the thromboxane A... receptor agonist U46619 [GenBank] (100 nM)-elicited contraction of coronary arteries. Thus a p47... and Src kinase activation of peroxide production by Nox2 appears to be an important contributor to vascular contractile mechanisms mediated through activation of PKC. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0363-6135
1522-1539