CCD imaging in cryospectrophotometric determination of microvascular oxyhemoglobin saturations

A microspectrophotometric imaging method has been developed for localized measurements of intravascular oxyhemoglobin (Hb02) saturations in microvessels from sections of quick-frozen tissue. Hb02 saturation was calculated from the absorption spectrum of red blood cells measured at five selected wave...

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Bibliographic Details
Published in:American journal of physiology. Heart and circulatory physiology 1997-12, Vol.42 (6), p.H2910-H2918
Main Authors: MASEIDE, K, ROFSTAD, E. K
Format: Article
Language:English
Subjects:
Anatomy & physiology
Biological and medical sciences
Blood
Blood vessels and receptors
Cardiovascular disease
Circulatory system
Fundamental and applied biological sciences. Psychology
Vertebrates: cardiovascular system
Online Access:Get full text
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Summary:A microspectrophotometric imaging method has been developed for localized measurements of intravascular oxyhemoglobin (Hb02) saturations in microvessels from sections of quick-frozen tissue. Hb02 saturation was calculated from the absorption spectrum of red blood cells measured at five selected wavelengths in the 520- to 570-nm range.
ISSN:0363-6135
1522-1539