Regulation of intracellular pH in proximal tubules of avian long-looped mammalian-type nephrons

In nonperfused proximal tubules isolated from chicken long-looped mammalian-type nephrons, intracellular pH (pHi), measured with the pH-sensitive fluorescent dye 2',7'-bis(2carboxyethyl)-5(6)-carboxyfluorescein, was tilde7.3 under control conditions (HEPES-buffered medium with pH 7.4 at 37...

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Bibliographic Details
Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1998-06, Vol.43 (6), p.R1526-R1535
Main Authors: BROKL, O. H, MARTINEZ, C. L, SHUPRISHA, A, ABBOTT, D. E, DANTZLER, W. H
Format: Article
Language:English
Subjects:
Anatomy & physiology
Biological and medical sciences
Birds
Fundamental and applied biological sciences. Psychology
Ions
Kidneys
Vertebrates: urinary system
Online Access:Get full text
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Summary:In nonperfused proximal tubules isolated from chicken long-looped mammalian-type nephrons, intracellular pH (pHi), measured with the pH-sensitive fluorescent dye 2',7'-bis(2carboxyethyl)-5(6)-carboxyfluorescein, was tilde7.3 under control conditions (HEPES-buffered medium with pH 7.4 at 37degC and was reduced to tilde7.0 in response to NH4Cl pulse.)
ISSN:0363-6119
1522-1490