Thermal acclimation of phase behavior in plasma membrane lipids of rainbow trout hepatocytes

The fluorescent probes laurdan (6-dodecanoyl-2-dimethylaminonapthalene) and N-A7-nitrobenz-2-oxa-1,3-diazol-4-ylB dipalmitoyl-L-alpha-phosphatidylethanolamine (NBD-PE) in addition to Fourier transform infrared spectroscopy (FTIR) were employed to measure the phase behavior and physical properties of...

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Bibliographic Details
Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1998-09, Vol.44 (3), p.R861-R869
Main Authors: HAZEL, J. R, MCKINLEY, S. J, GERRITS, M. F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fish
Fundamental and applied biological sciences. Psychology
Lipids
Membranes
Plasma
Temperature
Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:The fluorescent probes laurdan (6-dodecanoyl-2-dimethylaminonapthalene) and N-A7-nitrobenz-2-oxa-1,3-diazol-4-ylB dipalmitoyl-L-alpha-phosphatidylethanolamine (NBD-PE) in addition to Fourier transform infrared spectroscopy (FTIR) were employed to measure the phase behavior and physical properties of hepatocyte plasma membranes isolated from the livers of thermally acclimated (5 and 20degC) rainbow trout (Oncorhynchus mykiss).
ISSN:0363-6119
1522-1490