Inhibitory Activities on α-Glucosidase and Composition Analysis of Different Fractions of Ethanol Extracts from Actinidia chinensis Radix

• Published in: Chemical research in Chinese universities 2019-10, Vol.35 (5), p.823-829
• Main Authors: Nie, Changping, Yang, Jun, Wu, Dan, Wan, Luping, Liang, Guangping
• Format: Article
• Language: English
• Subjects: A15 compounds; Analytical Chemistry; Butanol; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Ethanol; Ethyl acetate; Glucosidase; Inorganic Chemistry; Liquid chromatography; Mass spectrometry; Natural products; Organic Chemistry; Physical Chemistry; Scientific imaging; Spectroscopy
• ISSN: 1005-9040; 2210-3171
• DOI: 10.1007/s40242-019-9037-1

The inhibitory actions of ethyl acetate, n -butanol and water fractions from Actinidia chinensis Radix (A. chinensis Radix) ethanol extracts on α -glucosidase were investigated. We found that the inhibitory activity of different ethanol extract fractions on α -glucosidase is concentration-dependent. At the same concentration, the ethyl acetate and n -butanol fractions showed higher inhibitory activities on α -glucosidase in comparison with acarbose. We then determined the chemical constituents of the ethyl acetate and n -butanol fractions through ultra performance liquid chromatography-triple-time of flight/mass spectrometry(UPLC-Triple-TOF/MS). Based on the mass spectrometry analysis and the databases Scifinder and Reaxys, 21 compounds(A 1 —A 21 ) were identified from the ethyl acetate fraction. In addition, 11 of those compounds(A 2 , A 3 , A 5 , A 6 , A 8 , A 10 , A 11 , A 13 , A 15 , A 19 , A 20 ) were identified for the first time from A. chinensis Radix. We also identified 14 compounds(A 22 —A 32 and A 4 —A 6 ) from the n -butanol fraction and compound A 29 is a new natural product. From the n -butanol fraction, 7 additional compounds (A 22 , A 25 , A 27 , A 28 , A 30 —A 32 ) were identified from A chinensis Radix for the first time.