Effect of Retinoic Acid on Apoptosis and DNA Repair in Human Keratinocytes after UVB Irradiation

Background: Skin cancer is extremely common. Epidemiological studies indicated that ultraviolet radiation (UV) is the primary cause for skin cancers, and that retinoic acid (RA) is able to inhibit this UV-induced skin carcinogenesis; however, the molecular mechanism of the anti-UV action of RA is un...

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Bibliographic Details
Published in:Journal of cutaneous medicine and surgery 2000-01, Vol.4 (1), p.2-7
Main Authors: Li, Gang, Bush, Jason A., Ho, Vincent C.
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
Apoptosis - drug effects
Biological and medical sciences
DNA Repair - drug effects
Humans
In Vitro Techniques
Keratinocytes - drug effects
Keratinocytes - radiation effects
Medical sciences
Pharmacology. Drug treatments
Skin, nail, hair, dermoskeleton
Tretinoin - pharmacology
Ultraviolet Rays
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Summary:Background: Skin cancer is extremely common. Epidemiological studies indicated that ultraviolet radiation (UV) is the primary cause for skin cancers, and that retinoic acid (RA) is able to inhibit this UV-induced skin carcinogenesis; however, the molecular mechanism of the anti-UV action of RA is unclear. Objective: The purpose of this study is to investigate if RA enhances the removal of UV-induced DNA damage. Methods: The effect of RA on UV-induced apoptosis and DNA repair was investigated by ELISA apoptosis assay and CAT assay. Results: Both all-trans-RA and 9-cis-RA did not promote UV-induced apoptosis nor the repair of UV-damaged DNA in human keratinocytes. Furthermore, RA did not induce the expression of p53. Conclusion: The inhibition of RA on skin carcinogenesis is not due to enhanced removal of UV-damaged DNA. Therefore, RA does not inhibit skin cancer development at the initiation stage, but possibly at the promotion and progression stages.
ISSN:1203-4754
1615-7109
DOI:10.1177/120347540000400102