Production of Stable Cell Lines on the Basis of the Cultured RPMI 8866 B-Cells with Constant and Inducible Expression of the Human Immunodeficiency Virus Tat Protein

Highly-efficient antiretroviral therapy allows controlling human immunodeficiency virus (HIV) and preventing the development of immunodeficiency. However, the patients who receive therapy may develop different complications, including B-cell lymphomas. One of oncogenesis’ mechanisms in HIV-infected...

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Bibliographic Details
Published in:Russian journal of developmental biology 2019-09, Vol.50 (5), p.275-280
Main Authors: Gorbacheva, M. A., Tikhomirova, M. A., Potashnikova, D. M., Akbay, B., Sheval, E. V., Musinova, Y. R.
Format: Article
Language:English
Subjects:
Animal Anatomy
Antiretroviral therapy
B-cell lymphoma
Biomedical and Life Sciences
Developmental Biology
Histology
HIV
Human immunodeficiency virus
Life Sciences
Lymphocytes B
Mechanisms of Cell Proliferation and Differentiation
Morphology
Proteins
Tat protein
Tumorigenesis
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Summary:Highly-efficient antiretroviral therapy allows controlling human immunodeficiency virus (HIV) and preventing the development of immunodeficiency. However, the patients who receive therapy may develop different complications, including B-cell lymphomas. One of oncogenesis’ mechanisms in HIV-infected patients is associated with the activity of the viral Tat protein, which is able to penetrate into B-cells. In order to study the effect of the Tat protein on B-cells, a report is given on the production and characterization of the cell lines based on the cultured RPMI 8866 B-cell line demonstrating constant and inducible expression of the Tat protein.
ISSN:1062-3604
1608-3326
DOI:10.1134/S1062360419050060