Elongation factor P controls translation of the mgtA gene encoding a Mg 2+ transporter during Salmonella infection

Ribosome often stalls on mRNA sequences harboring consecutive proline codons. Elongation factor P (EF-P) is required for the stalled ribosome to continue translation and thus the absence of EF-P affects translation of the associated open reading frame. Here we report that EF-P controls translation o...

Full description

Saved in:
Bibliographic Details
Published in:MicrobiologyOpen (Weinheim) 2019-04, Vol.8 (4), p.e00680
Main Authors: Choi, Eunna, Nam, Daesil, Choi, Jeongjoon, Park, Shinae, Lee, Jung-Shin, Lee, Eun-Jin
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Adenosine Triphosphatases - chemistry
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - metabolism
Amino Acid Motifs
Animals
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Codons
Elongation
Female
Gene Deletion
Gene Expression Regulation, Bacterial
Gene sequencing
Genes
Humans
Infections
Magnesium
Magnesium - metabolism
Membrane Transport Proteins - chemistry
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
Mice
mRNA
Pathogenesis
Peptide Elongation Factors - genetics
Peptide Elongation Factors - metabolism
Plasmids
Proline
Protein Biosynthesis
Proteins
Salmonella
Salmonella Infections - microbiology
Salmonella typhimurium - chemistry
Salmonella typhimurium - genetics
Salmonella typhimurium - metabolism
Translation
Translation elongation
Transportation
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ribosome often stalls on mRNA sequences harboring consecutive proline codons. Elongation factor P (EF-P) is required for the stalled ribosome to continue translation and thus the absence of EF-P affects translation of the associated open reading frame. Here we report that EF-P controls translation of the mgtA gene encoding a Mg -transporting ATPase from the intracellualr pathogen Salmonella enterica serovar Typhimurium. EF-P's effect on mgtA translation is dependent on the 550th and 551st proline codons in the coding region and thus substitution of those proline codons eliminates EF-P-mediated control of MgtA protein without affecting the Mg -transporting activity of the mgtA gene. The Pro550 and Pro551-substituted mgtA gene promotes Salmonella's intramacrophage survival and mouse virulence, suggesting that EF-P-mediated translational control of the mgtA gene is required for Salmonella pathogenesis.
ISSN:2045-8827
2045-8827
DOI:10.1002/mbo3.680