Immunoassay of plasmonic gold‐nanoparticle clusters: Plasmon coupling effects for Parkinson biomarker detection

A simple signal‐on plasmonic optical assay for the detection of the Parkinson biomarker using gold‐nanoparticle clusters (AuNCs) for signal amplification is presented. This approach is based on the improvement of the optical density (OD) change of the plasmonic band of a localized surface plasmon re...

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Bibliographic Details
Published in:Journal of the Chinese Chemical Society (Taipei) 2019-09, Vol.66 (9), p.982-987
Main Authors: Ko, Wen‐Yin, Chang, Chia‐Yu, Chiang, Yun‐Ting, Huang, Li‐Ting, Huang, Lih‐Wen, Lin, Kuan‐Jiuh
Format: Article
Language:English
Subjects:
Amplification
Antibodies
Au nanoparticle
Bioassays
Biomarkers
Clusters
Coupling
Diagnostic systems
Gold
Immunoassay
localized surface plasmon resonance
Nanoparticles
Optical communication
Optical density
Parkinson's disease
Plasmonic amplification
Sensitivity enhancement
α‐synuclein
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Summary:A simple signal‐on plasmonic optical assay for the detection of the Parkinson biomarker using gold‐nanoparticle clusters (AuNCs) for signal amplification is presented. This approach is based on the improvement of the optical density (OD) change of the plasmonic band of a localized surface plasmon resonance (LSPR) Au nanoparticle (AuNP) sensor interface using Au NCs conjugated antibodies. The amplification results in a 260‐fold improvement in concentration detection, from 1,000 ng/mL (unlabeled antibody) to 3.8 ng/mL (antibody‐conjugated AuNCs). The sensitivity enhancement can be ascribed to the further plasmonic coupling between the antibody‐conjugated AuNCs and the AuNPs on the LSPR interface and the enhanced amount of target molecule bound to the bioassay. This AuNCs‐assisted signal amplification strategy allows for improving the sensitivity of the plasmon‐based bioassays and can be extended to other optical‐based diagnostic technologies. Importantly, the simple detecting procedure and protocol assembly make it competitive with other existing sensing technologies such as ELISA, allowing for practical usage in clinical diagnostics. A simple signal‐on plasmonic optical assay for the detection of the Parkinson biomarker using gold‐nanoparticle clusters (AuNCs) for signal amplification is presented. The novelty of such an assay lies in its ultra‐low detection limit with the aid of optical density (OD) change.
ISSN:0009-4536
2192-6549
DOI:10.1002/jccs.201900072