AVP inhibits LPS- and IL-1beta-stimulated NO and cGMP via V1 receptor in cultured rat mesangial cells

The present study examined how arginine vasopressin (AVP) affects nitric oxide (NO) metabolism in cultured rat glomerular mesangial cells (GMC). GMC were incubated with test agents and nitrite, and intracellular cGMP content, inducible nitric oxide synthase (iNOS) mRNA, and iNOS protein were analyze...

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Bibliographic Details
Published in:American journal of physiology. Renal physiology 1999-03, Vol.45 (3), p.F433
Main Authors: Umino, Tetsuo, Kusano, Eiji, Muto, Shigeaki, Akimoto, Tetsu
Format: Article
Language:English
Subjects:
Anatomy & physiology
Kidneys
Rodents
Online Access:Get full text
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Summary:The present study examined how arginine vasopressin (AVP) affects nitric oxide (NO) metabolism in cultured rat glomerular mesangial cells (GMC). GMC were incubated with test agents and nitrite, and intracellular cGMP content, inducible nitric oxide synthase (iNOS) mRNA, and iNOS protein were analyzed by the Griess method, enzyme immunoassay, and Northern and Western blotting, respectively.
ISSN:1931-857X
1522-1466