Decreased abundance of urinary exosomal aquaporin-1 in renal ischemia-reperfusion injury

Urinary exosomes, secreted into urine from renal epithelial cells, are known to contain many types of renal functional membrane proteins. Here, we studied whether renal ischemia-reperfusion (I/R) affects urinary exosomal aquaporin-1 (AQP1) excretion in rats subjected to renal I/R and patients who un...

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Bibliographic Details
Published in:American journal of physiology. Renal physiology 2009-10, Vol.297 (4), p.F1006-F1016
Main Authors: Sonoda, Hiroko, Yokota-Ikeda, Naoko, Oshikawa, Sayaka, Kanno, Yosuke, Yoshinaga, Kazuya, Uchida, Kazuyuki, Ueda, Yuuji, Kimiya, Kouichi, Uezono, Shigehiro, Ueda, Akira, Ito, Katsuaki, Ikeda, Masahiro
Format: Article
Language:English
Subjects:
Animals
Antimetabolites, Antineoplastic
Aquaporin 1 - urine
Cells
Chediak-Higashi Syndrome - urine
Gene expression
Humans
Immunohistochemistry
Ischemia
Kidney - metabolism
Kidney diseases
Kidney Transplantation
Male
Proteins
Proteinuria - urine
Puromycin
Rats
Rats, Sprague-Dawley
Reperfusion Injury - blood
Reperfusion Injury - urine
RNA, Messenger - metabolism
Rodents
Transplants & implants
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Summary:Urinary exosomes, secreted into urine from renal epithelial cells, are known to contain many types of renal functional membrane proteins. Here, we studied whether renal ischemia-reperfusion (I/R) affects urinary exosomal aquaporin-1 (AQP1) excretion in rats subjected to renal I/R and patients who underwent renal transplantation. Immunoblotting studies demonstrated reduction of the urinary exosomal AQP1 level even at 6 h after renal I/R, and the level continued to be low over 96 h after I/R. Renal AQP1 mRNA and protein analyses revealed that the decreased excretion of urinary exosomal AQP1 is associated with renal AQP1 protein retention in the early phase and with a decreased expression level of renal AQP1 in the later phase of renal I/R injury. Decreased abundance of urinary exosomal AQP1 in a recipient patient was also observed at 48 h after renal allograft transplantation. No significant decrease in urinary exosomal AQP1 was observed in a rat model of nephropathy or in patients with proteinuria. Our studies suggest that the renal AQP1 expression level is possibly controlled by its urinary exosomal excretion and indicate that urinary exosomal AQP1 is a novel urinary biomarker for renal I/R injury.
ISSN:1931-857X
1522-1466
DOI:10.1152/ajprenal.00200.2009