The use of aggregation-induced emission probe doped silica nanoparticles for the immunoassay of human epididymis protein 4

A sensitive sandwich immunoassay for the sensing of human epididymis protein 4 (HE4) is developed based on aggregation-induced emission probe doped silica nanoparticles. Fluorescent silica nanoparticles (TSiO 2 ) with excellent performance were prepared using a tetraphenylethylene based probe (TPE-C...

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Published in:Analyst (London) 2019-10, Vol.144 (2), p.6136-6142
Main Authors: Meng, Lianjie, Nawaz, Muhammad Azhar Hayat, Huang, Xinan, Ma, Yuqin, Li, Yongxin, Zhou, Huipeng, Yu, Cong
Format: Article
Language:English
Subjects:
Agglomeration
Antibodies
Beads
Biosensing Techniques - methods
Cancer
Emission
Fluorescence
Fluorescent Dyes - chemistry
Humans
Immunoassay
Immunoassay - methods
Limit of Detection
Male
Nanoparticles
Nanoparticles - chemistry
Ovarian cancer
Proteins
Sensitivity and Specificity
Silicon dioxide
Silicon Dioxide - chemistry
Sodium salts
Stilbenes - chemistry
WAP Four-Disulfide Core Domain Protein 2 - analysis
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Summary:A sensitive sandwich immunoassay for the sensing of human epididymis protein 4 (HE4) is developed based on aggregation-induced emission probe doped silica nanoparticles. Fluorescent silica nanoparticles (TSiO 2 ) with excellent performance were prepared using a tetraphenylethylene based probe (TPE-C4-4) to produce a controlled aggregation effect, and HE4 was detected using antibody-functionalized fluorescent silica nanoparticles. The fluorescent silica nanoparticles possess good photophysical properties. The TSiO 2 NPs were surface modified with carboxyl groups, and the antibody was covalently attached onto the surface of the nanoparticles. In addition, magnetic beads were modified with N -hydroxysulfosuccinimide sodium salt (NHS) on their surface, capable of forming stable peptide bonds with the antibody. TSiO 2 NPs modified by an antibody and the magnetic beads modified by an antibody were used for the sandwich immunoassay for HE4 protein. The assay is quite sensitive and selective. The detection limit of HE4 is estimated to be 40 pM. The assay shows promising applications for the early diagnosis of ovarian cancer and the development of ovarian cancer-related drugs. A sensitive immunoassay for the sensing of human epididymis protein 4 is developed based on aggregation-induced emission probe doped silica nanoparticles.
ISSN:0003-2654
1364-5528
DOI:10.1039/c9an01265f