Debromination of the Flame Retardant Decabromodiphenyl Ether by Juvenile Carp (Cyprinus carpio) following Dietary Exposure

• Published in: Environmental science & technology 2004-01, Vol.38 (1), p.112-119
• Main Authors: Stapleton, Heather M, Alaee, Mehran, Letcher, Robert J, Baker, Joel E
• Format: Article
• Language: English
• Subjects: Administration, Oral; Agnatha. Pisces; Animal, plant and microbial ecology; Animals; Applied ecology; Bioavailability; Biological and medical sciences; Biological Availability; Bromobenzenes - metabolism; Bromobenzenes - pharmacokinetics; Carp; Carps - physiology; Chemical contaminants; Diet; Dose-Response Relationship, Drug; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Fire resistant materials; Fish; Flame retardants; Flame Retardants - metabolism; Flame Retardants - pharmacokinetics; Fundamental and applied biological sciences. Psychology; Halogenated Diphenyl Ethers; Liver - chemistry; Metabolites; Phenyl Ethers; Polybrominated Biphenyls; Studies; Tissue Distribution; Water Pollutants, Chemical - metabolism; Water Pollutants, Chemical - pharmacokinetics
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es034746j

The congener 2,2‘,3,3‘,4,4‘,5,5‘,6,6‘-decabromodiphenyl ether (BDE 209) is the primary component in a commonly used flame retardant known as decaBDE. This flame retardant constitutes approximately 80% of the world market demand for polybrominated diphenyl ethers (PBDEs). Because this compound is very hydrophobic (log K ow ∼ 10), it has been suggested that BDE 209 has very low bioavailability, although debromination to more bioavailable metabolites has also been suggested to occur in fish tis- sues. In the present study, juvenile carp were exposed to BDE 209 amended food on a daily basis for 60 days, fol- lowed by a 40-day depuration period in which the fate of BDE 209 was monitored in whole fish and liver tissues sepa- rately. No net accumulation of BDE 209 was observed throughout the experiment despite an exposure concentration of 940 ng/day/fish. However, seven apparent debrominated products of BDE 209 accumulated in whole fish and liver tissues over the exposure period. These debrominated metabolites of BDE 209 were identified as penta- to octaBDEs using both GC/ECNI-MS and GC/HRMS. Using estimation methods for relative retention times of phenyl substitution patterns, we have identified possible structures for the hexa- and heptabromodiphenyl ethers identified in the carp tissues. Although exposure of carp to BDE 209 did not result in the accumulation of BDE 209 in carp tissues, our results indicate evidence of limited BDE 209 bioavailability from food in the form of lower brominated metabolites.