Cardiac fibroblasts influence cardiomyocyte phenotype in vitro

1 Department of Pathology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Shadyside Hospital, Pittsburgh; and Departments of 2 Cardiothoracic Surgery, 3 Genomic Sciences, 4 Pathology, and 5 Pediatrics, Drexel University School of Medicine, Allegheny Genera...

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Bibliographic Details
Published in:American Journal of Physiology: Cell Physiology 2007-05, Vol.292 (5), p.C1799-C1808
Main Authors: LaFramboise, W. A, Scalise, D, Stoodley, P, Graner, S. R, Guthrie, R. D, Magovern, J. A, Becich, M. J
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Animals, Newborn
Cardiovascular system
Cell Differentiation
Cell Proliferation
Cell Shape
Cell Size
Cells
Cells, Cultured
Connexin 43 - metabolism
Culture Media, Conditioned - metabolism
Cytokines - metabolism
Fibroblasts - metabolism
Genotype & phenotype
Heart
Heart Ventricles - cytology
Heart Ventricles - metabolism
Myocardial Contraction
Myocytes, Cardiac - metabolism
Myocytes, Cardiac - ultrastructure
Myosin Heavy Chains - metabolism
Paracrine Communication
Phenotype
Proteomics
Rats
Rats, Sprague-Dawley
Rodents
Time Factors
Vimentin - metabolism
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Summary:1 Department of Pathology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Shadyside Hospital, Pittsburgh; and Departments of 2 Cardiothoracic Surgery, 3 Genomic Sciences, 4 Pathology, and 5 Pediatrics, Drexel University School of Medicine, Allegheny General Hospital, Pittsburgh, Pennsylvania Submitted 10 April 2006 ; accepted in final form 11 January 2007 Cardiac fibroblasts impact myocardial development and remodeling through intercellular contact with cardiomyocytes, but less is known about noncontact, profibrotic signals whereby fibroblasts alter cardiomyocyte behavior. Fibroblasts and cardiomyocytes were harvested from newborn rat ventricles and separated by serial digestion and gradient centrifugation. Cardiomyocytes were cultured in 1 ) standard medium, 2 ) standard medium diluted 1:1 with PBS, or 3 ) standard medium diluted 1:1 with medium conditioned 72 h by cardiac fibroblasts. Serum concentrations were held constant under all media conditions, and complete medium exchanges were performed daily. Cardiomyocytes began contracting within 24 h at clonal or mass densities with
ISSN:0363-6143
1522-1563
DOI:10.1152/ajpcell.00166.2006