Inducible expression of erythrocyte band 3 protein

A permanent cell line with inducible expression of the human anion exchanger protein 1 (hAE1) was constructed in a derivative of human embryonic kidney cells (HEK-293). In the absence of the inducer, muristerone A, the new cell line had no detectable hAE1 protein by Western analysis or additional Cl...

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Bibliographic Details
Published in:American Journal of Physiology: Cell Physiology 1999, Vol.45 (1), p.C66-C75
Main Authors: TIMMER, R. T, GUNN, R. B
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Anatomy & physiology
Binding and carrier proteins
Biological and medical sciences
Cells
Fundamental and applied biological sciences. Psychology
Kidneys
Kinetics
Proteins
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Summary:A permanent cell line with inducible expression of the human anion exchanger protein 1 (hAE1) was constructed in a derivative of human embryonic kidney cells (HEK-293). In the absence of the inducer, muristerone A, the new cell line had no detectable hAE1 protein by Western analysis or additional Cl flux. Increasing dose and incubation time with muristerone A increased the amount of protein (both unglycosylated and glycosylated).
ISSN:0363-6143
1522-1563