Granulocyte colony-stimulating factor promotes adhesion of neutrophils

• Published in: American Journal of Physiology: Cell Physiology 2003, Vol.53 (1), p.C103-C110
• Main Authors: CHAKRABORTY, Arup, HENTZEN, Eric R, SEO, Scott M, SMITH, C. Wayne
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Blood vessels and receptors; Cells; Fundamental and applied biological sciences. Psychology; Neurotransmitters; Vertebrates: cardiovascular system
• ISSN: 0363-6143; 1522-1563

Granulocyte colony stimulating factor (G-CSF) is well known for its ability to drive the maturation and mobilization of neutrophils. G-CSF also appears to have the potential to activate functions of mature neutrophils, influencing recruitment at sites of inflammation and tissue injury. We investigated the ability of G-CSF to stimulate adhesion of isolated blood neutrophils. G-CSF induced significant adherence to intercellular adhesion molecule (ICAM)-1 that was both macrophage antigen-1 (Mac-1) and leukocyte function-associated antigen-1 dependent. The kinetics of G-CSF-stimulated adhesion to ICAM-1 peaked at 11 min without detectable surface upregulation of Mac-1. This was in marked contrast to chemokines, in which peak activation of adhesion is seen within 1 min of stimulation. In contrast to chemokine-induced adhesion, G-CSF stimulation was not inhibited by pertussis toxin. G-CSF also augmented the attachment of neutrophils to activated human umbilical vein endothelial cells (HUVEC) through specific effects on neutrophils, because HUVEC appear to lack functional G-CSF receptors.