Analysis of eEF1Bγ interactome in the nuclear fraction of A549 human lung adenocarcinoma cells

Aim. To study the translation elongation factor eEF1B gamma (eEF1Bγ) in the nucleus of lung carcinoma cells. Methods.The protein partners of eEF1Bγ in the nuclear fraction of A549 cells were identified by co-immunoprecipitation (co-IP) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). T...

Full description

Saved in:
Bibliographic Details
Published in:Biopolimery i kletka 2019, Vol.35 (4), p.268-287
Main Authors: Kapustian, L. M., Lysetsky, I. L., Bondarchuk, T. V., Novosylna, O. V., Negrutskii, B. S.
Format: Article
Language:English
Subjects:
Adenocarcinoma
Carcinogenesis
Cytoplasm
Immunoprecipitation
Liquid chromatography
Lung cancer
Lung carcinoma
Mass spectroscopy
mRNA stability
Splicing
Translation elongation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aim. To study the translation elongation factor eEF1B gamma (eEF1Bγ) in the nucleus of lung carcinoma cells. Methods.The protein partners of eEF1Bγ in the nuclear fraction of A549 cells were identified by co-immunoprecipitation (co-IP) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The protein interaction network for nuclear eEF1Bγ was determined by Cytoscape 3.2.0 program using the MCODE plugin. Additional analysis of the eEF1Bγ partners was conducted by Map of the cell database. Results. 234 proteins interacting with eEF1Bγ in the nuclear fraction of A549 cells were identified. Possible functional networks involving these contacts were analyzed by two bioinformatic approaches. Conclusions. Splicing of pre-mRNA and regulation of mRNA stability are assumed to be the main processes in which nuclear eEF1Bγ can be involved. We hypothesize that a portion of eEF1Bγ leaves the cytoplasm-localizede EF1B complex during carcinogenesis and enters the nucleus to regulate certain mRNAs by affecting the splicing of their pre-mRNA and/or stability of mRNA.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.000A0A