Application of silicalite for improvement of enzyme adsorption on the stainless steel electrodes

Aim. Improvement of analytical characteristics of an enzyme biosensor based on new inexpensive perspective stainless steel electrodes using silicalite nanoparticles. Methods. Conductometric enzyme biosensor was used. Results. Three methods of glucose oxidase (GOx) immobilization were studied and com...

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Bibliographic Details
Published in:Biopolimery i kletka 2014, Vol.30 (6), p.462-468
Main Authors: Pyeshkova, V. N., Dudchenko, O. Y., Soldatkin, O. O., Kucherenko, I. S., Ozansoy Kasap, B., Akata Kurc, B., Dzyadevych, S. V.
Format: Article
Language:English
Subjects:
Adsorption
Biosensors
Electrodes
Enzymes
Glucose oxidase
Glutaraldehyde
Immobilization
Nanoparticles
Shelf life
Stainless steel
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Summary:Aim. Improvement of analytical characteristics of an enzyme biosensor based on new inexpensive perspective stainless steel electrodes using silicalite nanoparticles. Methods. Conductometric enzyme biosensor was used. Results. Three methods of glucose oxidase (GOx) immobilization were studied and compared: GOx adsorption on silicalite modified electrodes (GOx-SME); cross-linking by glutaraldehyde without silicalite (GOx-GA); GOx adsorption on SME along with cross-linking by glutaraldehyde (GOx-SME-GA). The GOx-SME-GA biosensors based on stainless steel electrodes were characterized by 12–25-fold higher sensitivity comparing with other biosensors. The developed GOx-SME-GA biosensors were characterized by good reproducibility of glucose biosensors construction (relative standard deviation (RSD) – 18 %), improved signal reproducibility (RSD of glucose determination was 7 %) and good storage stability (29 % loss of activity after 18 days). Conclusions. The method of enzyme immobilization using silicalite together with GA cross-linking sufficiently enhances the enzyme adsorption on the stainless steel electrodes and improves the analytical parameters of biosensors. This method is found to be promising for further creation of other enzyme biosensors.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0008C1