Population dynamics of Oenococcus oeni strains in a new winery and the effect of SO2 and yeast strain

• Published in: FEMS microbiology letters 2005-05, Vol.246 (1), p.111-117
• Main Authors: Reguant, C, Carrete, R, Constanti, M, Bordons, A
• Format: Article
• Language: English
• Subjects: alcoholic fermentation; Biological and medical sciences; Biotechnology; DNA Fingerprinting; DNA, Bacterial - analysis; DNA, Bacterial - isolation & purification; Fermentation; Food Microbiology; Food processing; Fundamental and applied biological sciences. Psychology; Gram-Positive Cocci - classification; Gram-Positive Cocci - growth & development; Gram-Positive Cocci - isolation & purification; Gram-Positive Cocci - metabolism; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Industrial applications and implications. Economical aspects; lactic acid bacteria; Malolactic fermentation; Microbiology; Mycology; Oenococcus oeni; Population dynamics; Random amplified polymorphic DNA; Random Amplified Polymorphic DNA Technique; RAPD multiplex; starter cultures; Starters; strain identification; Strain typification; strains; Sulfur Dioxide; Sulphur dioxide; Wine; Wine - microbiology; wine yeasts; winemaking; Wineries; Wineries & vineyards; wines; Yeast; Yeasts
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/j.femsle.2005.03.045

Abstract The effects of different yeast starters and SO2 addition on malolactic fermentation in a new winery were evaluated by a molecular approach in three vintages. Alcoholic fermentations with 40 and 100 mg l−1 SO2 were carried out, followed by uninoculated malolactic fermentations. Isolated colonies of Oenococcus oeni obtained from samples throughout the vinification were identified and typified by multiplex RAPD-PCR. This made it possible to monitor the population dynamics and follow the proportion of as many as 29 different indigenous strains. In one of the vintages, O. oeni strains were more inhibited when a specific yeast starter was used.